Objective: Hyperlipidemia is one of the risk factors that contribute to the prevalence of coronary heart diseases and antihyperlipidemic agents, such as statin, was used to treat hyperlipidemia as a current therapy. Boesenbergia pandurata has not been exploited for antihyperlipidemic effect. Hence, this study aims to screen for the antihyperlipidemic activity of methanolic extracts of B. pandurata rhizomes (BPR extracts) in hypercholesterolemia-induced Sprague-Dawley rats.Methods: BPR extracts were prepared using the maceration method with 1500 ml of 80% methanol at room temperature for about 7 days. A toxicity study was carried out based on OECD guidelines. Hypercholesterolemia was induced by 6% lard oil, 2% of cheese, and egg yolks. Two different doses of BPR extracts, 200 and 400 mg/kg, were used to screen for antihyperlipidemic effect. Histopathological study was carried out in the liver. The results were evaluated for the statistically significant difference by using the one-way ANOVA followed by post hoc Dunnett test.Results: No mortality was witnessed even till 2 g/kg. Only 400 mg/kg of BPR extracts statistically reduced in total cholesterol (p<0.05), low-density lipoprotein-cholesterol (p<0.05) and an increase in high-density lipoprotein-cholesterol (p<0.05) when compared to the positive control. BPR extracts (400 mg/kg) showed less enlargement of lipid droplets as compared to positive control.Conclusion: BPR extracts can be a promising medicinal plant for treating hyperlipidemia in underdeveloped countries.
In this work, four cyanuric chloride based chiral reagents were prepared via nucleophile substitution
of chlorine atom by L-proline derivatives and characterized by UV, FT-IR, HRMS, NMR and elemental
analysis. Racemic propranolol was chosen for the chiral recognition study. The prepared chiral reagents
were used in the synthesis of diastereomeric derivatives of (RS)-propranolol, under microwave heating
conditions. RP-HPLC was used to separate the prepared diastereomeric derivatives. The effect of
varying eluting phase concentrations and sample concentrations was optimized. The DFT calculations
were performed using Gaussian 09 Rev A.02 to create the lowest energy optimised structures of
diastereomeric derivatives. LOD (0.324 ng mL-1), LOQ (0.972 ng mL-1), calibration range (0.02-2.0
mg mL-1), correlation-coefficient (0.999), and recovery were the validation parameters for the present
method (99.09 and 99.81 % for inter-day assay and 98.47 and 99.72 % for intra-day assay).
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