Bioretention cells are popular stormwater management systems for controlling peak runoff and improving runoff water quality. A case study on a functional large-scale bioretention cell and a laboratory column experiment was conducted to evaluate the concentrations and retention efficiency of bioretention cells towards tire wear particles (TWP). The presence of TWP was observed in all soil fractions (<50 µm, 50–100 µm, 100–500 µm, and >500 µm) of the functional bioretention cell. TWP concentrations were higher (30.9 ± 4.1 mg/g) close to the inlet to the bioretention cell than 5 m away (19.8 ± 2.4 mg/g), demonstrating the influence of the bioretention cell design. The column experiment showed a high retention efficiency of TWP (99.6 ± 0.5%) in engineered soil consisting of sand, silty-sand, and garden waste compost. This study confirmed that bioretention cells built with engineered soil effectively retained TWP > 25 µm in size, demonstrating their potential as control measures along roads.