Glycopolymers carrying sulfated saccharides with modest sugar contents (11% and 28%) were found to suppress the formation of amyloid fibrils by amyloid beta peptides (Abeta(1-42), Abeta(1-40), and Abeta(25-35)), as evaluated by thioflavin T assays and atomic force microscopy observation. Circular dichroism spectra showed that the conformation of amyloid beta peptides depended on the glycopolymer additives, and that the glycopolymer additives reduced the beta-sheet contents. Neutralization activity was confirmed by in vitro assay with HeLa cells. The sulfate group and the appropriate sugar contents were essential for the inhibitory effect.
Glycopolymers carrying sulfate saccharides were found to suppress the formation of
amyloid fibrils by amyloid beta peptides, as evaluated by fluorescence assay of thioflavin T and AFM.
CD spectra showed that the conformation of amyloid beta peptides was changed from beta peptides
depended on the glycopolymer additives, and that the glycopolymer additives reduced the β-sheet
contents. Neutralization activity was confirmed by in vitro assay with HeLa cells. The sulfate group
and the appropriate sugar contents were essential for the inhibitory effect.
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