No abstract

No abstract

The effects of daylength, irradiance and spore settlement density on the growth, maturation and sporophyte production of Undaria pinnatifida (Harvey) Suringar gametophytes were examined using a factorial experimental design in culture. The growth of Undaria gametophytes increased with increasing daylength (8, 12 and 16 h), but the maximum fertility occurred at a daylength of 12 h followed by 8 and 16 h. Gametophytes grew better at the 16 h daylength under the same mean daily irradiance (MDI) of 20 µmol photons m −2 s −1 . However, the fertility was higher at the short daylength (8 h), indicating that the maturation of U. pinnatifida gametophytes is influenced by daylength rather than by the MDI. Vegetative growth and sporophyte production of gametophytes were better at 60 µmol photons m −2 s −1 than at 30 µmol photons m −2 s −1 under a 8:16 h LD (Light: Dark) cycle, and their growth and maturation were density-dependant in 16 and 12 h daylength, respectively. These results suggest that the U. pinnatifida gametophytes require a certain amount of light for the growth and reproduction, and intraspecific competition occurred under the optimal growth and maturation conditions. However, the sporophyte recruits per unit has been enhanced with increasing spore settlement density at 8 and 12 h daylengths indicating that high settlement density gives a benefit for maintaining population, even though the sporophyte production of each female plant is inhibited. In conclusion, the vegetative growth, reproduction and sporophyte production of U. pinnatifida gametophytes are retarded at a low irradiance above growth saturation and a high settlement density, and are determined by daylength.

Two species of Osmundea Stackhouse (Rhodomelaceae, Rhodophyta) that occur in Atlantic Europe have been confused under the names Osmundea ramosissima (Oeder) Athanasiadis and Osmundea truncata (Kützing) Nam et Maggs, regarded until now as a synonym of O. ramosissima. An epitype from its type locality (Stavanger, Norway) is selected for Osmundea ramosissima Athanasiadis, recognized here as a valid name for Fucus ramosissimus Oeder, nom. illeg. Details of vegetative and reproductive morphology of O. ramosissima are reported, based on material from France, the British Isles, and Helgoland. Osmundea ramosissima resembles other species of Osmundea in its vegetative axial segments with two pericentral cells and one trichoblast, spermatangial development from apical and epidermal cells (filament type), the formation of five pericentral cells in the procarp-bearing segment of the female trichoblast, and tetrasporangial production from random epidermal cells. Among the species of Osmundea, O. ramosissima is most similar to O. truncata. Both species have discoid holdfasts, secondary pit connections between epidermal cells, and cup-shaped spermatangial pits. They differ in that: (a) O. ramosissima lacks lenticular wall thickenings and refractive needle-like inclusions in medullary cells, both of which are present in O. truncata; (b) O. ramosissima has branched spermatangial filaments that terminate in a cluster of several cells, whereas in O. truncata the unbranched spermatangial filaments have a single large terminal sterile cell; and (c) cystocarps of O. ramosissima lack protuberant ostioles but ostioles are remarkably protuberant in O. truncata. Phylogenetic analyses of rbcL sequences of Laurencia obtusa (Hudson) Lamouroux and all five Atlantic European species of Osmundea, including the type species, strongly support the generic status of Osmundea. Osmundea ramosissima and O. truncata are closely related (5.2% sequence divergence) and form a well-supported clade sister to a clade consisting of O. pinnatifida (Hudson) Stackhouse, O. osmunda Stackhouse and O. hybrida (A. P. de Candolle) Nam. The formation of secondary pit connections between epidermal cells is a synapomorphy for the O. ramosissima+O. truncata clade. The close relationship between species with cup-shaped spermatangial pits (Osmundea hybrida) and urn-shaped pits (Osmundea pinnatifida and Osmundea osmunda) shows that spermatangial pit shape is not an important phylogenetic character. Parsimony analysis of a morphological data set also supports the genus Osmundea but conflicts with the molecular trees in infrageneric relationships, placing O. hybrida basal within the Osmundea clade and grouping O. osmunda and O. pinnatifida but not O. truncata and O. ramosissima. A key to Osmundea species is presented.

Laurencia succulenta sp. nov. (Rhodophyta) is described from Korea. This species exhibits vegetative and reproductive structures typical of the genus, but is distinct from similar species in its epiphytic habitat and the fleshy, robust, thick and subcompressed thalli with basically distichous branching. In addition, it is readily distinguished from the most similar species, such as L. nipponica Yamada and L. okamurae Yamada, by the cystocarps with a somewhat protuberant ostiole. In a phylogenetic analysis of 47 species of the Laurencia Lamouroux complex from various localities around the world based on 49 morphological characters, four major clades (Laurencia, Chondrophycus palisadus (Yamada) Nam group, C. cartilagineus (Yamada) Garbary et Harper group and Osmundea Stackhouse assemblage), each of which forms a monophyletic group, were recognized. Among these, the Laurencia clade is basal to the overall assemblage, and is defined by the vegetative axis with four rather than two pericentral cells. The Osmundea clade is supported by autapomorphic characters for the genus, features associated with spermatangial formation of the filament type and tetrasporangial production from epidermal cells. By contrast, Chondrophycus, a genus characterized by a combination of features (vegetative axis with two pericentral cells, trichoblast-type spermatangial development and tetrasporangial production from pericentral cells), is paraphyletic, and the species were separated into two well-supported clades, the C. palisadus group and C. cartilagineus group. These clades are distinguished from each other by the position of the first pericentral cell relative to the trichoblast, the presence or absence of fertility at the second pericentral cells and number of sterile pericentral cells in the tetrasporangial axis, the pattern of formation of spermatangial branches on trichoblasts, post-fertilization feature associated with the formation time of the auxiliary cell, and, probably, the number of pericentral cells in the procarp-bearing segment. Of these features, the side position of the first pericentral cell in the latter group (a synapomorphy for the C. cartilagineus group plus Osmundea) suggests that the C. cartilagineus group is more closely related to Osmundea than to the C. palisadus group. This cladistic analysis indicates that Chondrophycus is not monophyletic, suggesting that the C. palisadus group should be separated from Chondrophycus at the genus level. Based on this result, Palisada (Yamada) stat. nov. is proposed for the group, together with an emendation of the generic delineation of Chondrophycus, and relevant nomenclatural changes for several Chondrophycus species are also included. In addition, Corynecladia J. Agardh is reinstated for the type species L. clavata Sonder.

The effects of temperature, irradiance, and daylength on Sargassum horneri growth were examined at the germling and adult stages to discern their physiological differences. 15, 20, 25, 30°C× 20, 40, 80 ) and 13% day −1 (8 h daylength). In contrast, the RGRs of the blade weights were 4% day −1 (15°C, 20 μmol photons m −2 s −1 ) and 5% day −1 (12 h daylength). Negative growth rates were found at 20 μmol photons m −2 s −1 of 20°C and 25°C treatments after 12 days. This phenomenon coincides with the necrosis of S. horneri blades in field populations.In conclusion, we found physiological differences between S. horneri germlings and adults with respect to daylength and temperature optima. The growth of S. horneri germlings could be enhanced at 25°C, 20 μmol photons m , and 8 h daylength for construction of Sargassum beds and restoration of barren areas.

No abstract

A new antibacterial dioxopiperazine, dehydroxybisdethiobis(methylthio)gliotoxin (1), and the previously described bisdethiobis(methylthio)gliotoxin (2) and gliotoxin (3), have been isolated from the broth of a marine-derived fungus of the genus Pseudallescheria. The structure and absolute stereochemistry of the new compound was assigned on the basis of NMR and CD experiments. Compounds 1ϳ3 exhibit potent antibacterial activity against the methicillin-resistant and multidrugresistant Staphylococcus aureus with MIC values of 31.2, 31.2, and 1.0 m g/ml, respectively. Compound 3 also exhibited a significant radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) with IC 50 value of 5.2 mM.