This study was conducted from October 2019 to October 2020.To determine the prevalence of Entamoeba histolytica and Giardia lamblia among patients attending some hospitals (22 May Hospital, Cuban Hospital, Al-Naqeeb Hospital, Al Salam Hospital) in Al-manssora Directorate, Aden - Yemen. This study included 603 patients. Stool samples were obtained from all participants and laboratory conducted using a direct mount preparation with physiological normal saline (0.9%) and iodine stain. The results of the current study showed that the overall prevalence rate was 384/603 (63.68%). The prevalence of E. histolytica and G. lamblia was higher among males (33.34%) than that in female (30.34%). The prevalence rate of these parasites was higher in the age group between 20-67 years old (36.31%), followed by the age group (10-19) years. The study concluded that the prevalence of Entamoeba histolytica and Giardia lamblia among diarrheal patients in Al-manssora Directorate, Aden – Yemen was high
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