Nitrogen is one of the major growth-limiting nutrients for plants: The main source of nitrogen in most of the higher plants is nitrate taken up through roots. Nitrate
can be reduced both in the chloroplasts (photosynthetic tissues) and in proplastes (nonphotosynthetic tissues) such as roots. Ferredoxin-nitrite reductase (NiR)
catalyses the reduction of nitrite to ammonium in the second step of the nitrate- assimilation pathway. Homology model of Ferredoxin-nitrite reductase has been
constructed using the X-ray structure (PDB code: 2akj) a s a template and MODELLER 9v5 software. The resulting model assessed by PROCHECK, PROSAII
and RMSD that showed the final refined model is reliable: has 81% of amino acid sequence identity with template, 0.2Å as RMSD and has (-10.37) as Z-scores,
the Ramachandran plot analysis showed that conformations for 99.5 % of amino acid residues are within the most favored regions. The model could prove useful
in further functional characterization of this protein.AbbreviationsPDB - Protein Data Bank,
NMR - Nuclear Magnetic Resonance,
NiR - Nitrite Reductase,
RMSD - Root Mean Squared Deviation,
Fd - ferredoxin.
A field experiment was conducted on a private farm in El Oued State of Algeria to study the efficiency of using Fertil Verde as a fertilizer by foliar spry on hot pepper seedlings. The treatment by Fertil Verde increased all growth. The increase was significant in the number of main and secondary stems, the size of the leaves, and the weight of the dry leaves. While, the weight of the wet leaves did not significantly increase. Moreover, the use of Fertil Verde has led to a significant increase in the yield qualities of the plant: the weight and shape of the fruits, fresh yield of the plant, the content of pigment, and the size of the plant. Thus, the combination of fertilizing is common and Fertil Verde is a promising low-cost option in the production of high yields.
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