Experiments were conducted to determine optimal salinity, temperature, nutrient level and CO 2 supply required for achieving maximal growth in culture of two commercially important microalgae Nannochloropsis gaditana L.M. Lubián and Dunaliella salina (Dunal) Teodoresco. Four different salinities, temperatures and nutrient levels (NaNO 3) and two different CO 2 levels were employed. The results indicate that maximum cell density was obtained in N. gaditana with cultures in 25 ppt salinity, 25°C temperature, 150 g/lit nutrient level and 0.5 scfm CO 2 supply. In the case of D. salina the highest cell density was with 150 ppt salinity, 30°C temperature, 150 g/lit nutrient level and 2 scfm CO 2 supply. Second set of experiments with a combination of optimal parameters resulted in significant increase in cell density with both algae compared to routine culture conditions.
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