The study was conducted to investigate the effect of Humic Acid organic fertilizer on the performance of Thymus Kotschyanus populations under the influence of drought stress. This experiment was performed as a three-factor factorial in the form of a completely randomized design (CRD) for 5 replications (pots) in the greenhouse of Pardisan Research Station belonging to Qom Agricultural and Natural Resources Research and Training Center. Three factors include 5 top accessions of T. Kotschyanus (factor A), irrigation levels at 80% crop capacity (control), irrigation at 60% crop and irrigation capacity at 40% crop capacity (FC) (factor B); and different levels of Humic acid including 3 Levels of 0, 1500 and 3000 mg/l were dissolved in irrigation water twice after full establishment of the plants and twenty days after the first iteration (factor C). The results of trait variance analysis showed that there was a significant difference at the level of possible error of 1% or 5% between the top accessions of T. Kotschyanus and between different irrigation levels in terms of all traits under study. Effectiveness of accession × irrigation levels were also significant in most traits at the level of 1% or 5% probability of error. In other words, the behavior of different Thymus accessions at different irrigation levels was significantly different and the effect of irrigation levels on different irrigations was not similar. These results are consistent with the reports of many researchers on the effect of drought stress on morphological traits as well as increasing essential oil content in drought stress conditions in medicinal plants. Hassani et al. (2006) reported on the effect of different levels of water stress on growth, performance, and essential oil content of Dracocephalum moldavica. Many traits, such as aerial organ length, wet and dry weight of aerial organ were reduced in dry stress conditions, and the highest percentage of essential oil was obtained for 70% crop capa
Background: Global climate change and associated adverse abiotic stress conditions, such as drought, salinity, heavy metals, waterlogging, extreme temperatures, oxygen deprivation, etc., greatly influence plant growth and development, ultimately affecting crop yield and quality, as well as agricultural sustainability in general. This study provides new insights into the analysis of the function of soybean genes in abiotic stress. Drought is one of the significant constraints that limit agricultural productivity. Some factors, including climate changes and acreage expansion, indicate the need for developing drought-tolerant Genotypes.Materials and methods: The study of the expression Glutathione Reductase (GR) gene in soybean drought-tolerant and sensitive cultivars using real-time PCR. Seeds from (drought-sensitive) and (drought-tolerant) lines were planted under specific temperature conditions drought stress treatment, in the research greenhouse of Islamic Azad University of Arak, Iran. Changes in gene expression compared to reference genes were recorded using the formula 2-ΔΔCT. Three technical replications were given for each cDNA sample related to each sampling and used to analyze test data from MINITAB16 software.Results: The results showed that the threshold expression of gene expression (Glutathione) in the Pyramid line had the highest expression of drought resistance and the lowest expression of the Glutathione Reductase gene belonging to the Will line. The theory is also true that chaperone proteins produced during the plant growth cycle are not destroyed to express the Glutathione Reductase gene. The expression cycle of the Glutathione Reductase gene shows that the proteins produced by this gene have a high rate of expression and increase in cell drought stress. This gene expression continues until the pressure ends. The results showed that lines and cultivars with a weak expression against drought stress could have a high expression at the beginning of drought stress but a decrease in gene expression rate during stress. Drought stress-sensitive lines have a decreasing expression in the middle and end of stress during the stress period.
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