Over the last decade, an increasing number of studies have used lake sediment DNA to trace past landscape changes, agricultural activities or human presence. However, the processes responsible for lake sediment formation and sediment properties might affect DNA records via taphonomic and analytical processes. It is crucial to understand these processes to ensure reliable interpretations for “palaeo” studies. Here, we combined plant and mammal DNA metabarcoding analyses with sedimentological and geochemical analyses from three lake-catchment systems that are characterised by different erosion dynamics. The new insights derived from this approach elucidate and assess issues relating to DNA sources and transfer processes. The sources of eroded materials strongly affect the “catchment-DNA” concentration in the sediments. For instance, erosion of upper organic and organo-mineral soil horizons provides a higher amount of plant DNA in lake sediments than deep horizons, bare soils or glacial flours. Moreover, high erosion rates, along with a well-developed hydrographic network, are proposed as factors positively affecting the representation of the catchment flora. The development of open and agricultural landscapes, which favour the erosion, could thus bias the reconstructed landscape trajectory but help the record of these human activities. Regarding domestic animals, pastoral practices and animal behaviour might affect their DNA record because they control the type of source of DNA (“point” vs. “diffuse”).

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This paper presents and discusses the use of soil charcoal analysis (pedoanthracology) to reconstruct past forest dynamics in a larch forest of the upper Guil valley (French Alps, Queyras). We also discuss the role of anthropogenic fire in forest dynamics. The radiocarbon dates from this site demonstrate that arolla pine ( Pinus cembra) and larch were present in the area since 7566—7673 cal. BP and 5934—6123 cal. BP, respectively. The identification of a piece of charcoal to fir ( Abies alba) dated to 5734—5908 cal. BP suggests that this species was present at 1980 m a.s.l. since c. 6000 cal. BP and had a higher ecological tolerance than previously assumed. The cover of larch forests has increased since the second half of the Holocene because of anthropogenic practices (eg, clearing with fire, cattle grazing). However, today, owing to the abandonment of pastoral practices, the arolla pine has become dominant, which greatly modifies the forest structure and the landscape.

Over the last decade, an increasing number of studies have used lake sediment DNA to trace past landscape changes, agricultural activities or human presence. However, the processes responsible for lake sediment formation might affect DNA archiving via taphonomic and analytical processes. It is crucial to understand these processes to ensure reliable interpretations for “palaeo” studies. Here, we combined plant and mammal DNA metabarcoding analyses with sedimentological and geochemical analyses from three lake-catchment systems that are characterised by different erosion dynamics. The new knowledge from this approach concern the DNA sources and transfer processes. The sources of eroded materials strongly affect the “catchment-DNA” concentration in the sediments. For instance, erosion of upper organic and organo-mineral soil horizons provides higher amount of plant DNA in lake sediments than deep horizons, bare soils or glacial flours. Moreover, high erosion rates along with a well-developed hydrographic network, are proposed as factors positively affecting the representation of the catchment flora. The development of open and agricultural landscapes, which favour the erosion, could thus bias the reconstructed landscape trajectory. Regarding domestic animals, pastoral practices and animal behaviour might affect their DNA record because they control the type of source of DNA (“point” vs. “diffuse”).

As part of the Changing the Face of the Mediterranean Project, we consider how human pressure and concomitant erosion has affected a range of Mediterranean landscapes has between the Neolithic

The European Alps are highly rich in species, but their future may be threatened by ongoing changes in human land use and climate. Here, we reconstructed vegetation, temperature, human impact and livestock over the past ~12,000 years from Lake Sulsseewli, based on sedimentary ancient plant and mammal DNA, pollen, spores, chironomids, and microcharcoal. We assembled a highly-complete local DNA reference library (PhyloAlps, 3923 plant taxa), and used this to obtain an exceptionally rich sedaDNA record of 366 plant taxa. Vegetation mainly responded to climate during the early Holocene, while human activity had an additional influence on vegetation from 6 ka onwards. Land-use shifted from episodic grazing during the Neolithic and Bronze Age to agropastoralism in the Middle Ages. Associated human deforestation allowed the coexistence of plant species typically found at different elevational belts, leading to levels of plant richness that characterise the current high diversity of this region. Our findings indicate a positive association between low intensity agropastoral activities and precipitation with the maintenance of the unique subalpine and alpine plant diversity of the European Alps.

Agricultural terraced landscapes, which are important historical heritage sites (e.g., UNESCO or Globally Important Agricultural Heritage Systems (GIAHS) sites) are under threat from increased soil degradation due to climate change and land abandonment. Remote sensing can assist in the assessment and monitoring of such cultural ecosystem services. However, due to the limitations imposed by rugged topography and the occurrence of vegetation, the application of a single high-resolution topography (HRT) technique is challenging in these particular agricultural environments. Therefore, data fusion of HRT techniques (terrestrial laser scanning (TLS) and aerial/terrestrial structure from motion (SfM)) was tested for the first time in this context (terraces), to the best of our knowledge, to overcome specific detection problems such as the complex topographic and landcover conditions of the terrace systems. SfM–TLS data fusion methodology was trialed in order to produce very high-resolution digital terrain models (DTMs) of two agricultural terrace areas, both characterized by the presence of vegetation that covers parts of the subvertical surfaces, complex morphology, and inaccessible areas. In the unreachable areas, it was necessary to find effective solutions to carry out HRT surveys; therefore, we tested the direct georeferencing (DG) method, exploiting onboard multifrequency GNSS receivers for unmanned aerial vehicles (UAVs) and postprocessing kinematic (PPK) data. The results showed that the fusion of data based on different methods and acquisition platforms is required to obtain accurate DTMs that reflect the real surface roughness of terrace systems without gaps in data. Moreover, in inaccessible or hazardous terrains, a combination of direct and indirect georeferencing was a useful solution to reduce the substantial inconvenience and cost of ground control point (GCP) placement. We show that in order to obtain a precise data fusion in these complex conditions, it is essential to utilize a complete and specific workflow. This workflow must incorporate all data merging issues and landcover condition problems, encompassing the survey planning step, the coregistration process, and the error analysis of the outputs. The high-resolution DTMs realized can provide a starting point for land degradation process assessment of these agriculture environments and supplies useful information to stakeholders for better management and protection of such important heritage landscapes.

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