East Lansing, MI 48824 Cyclic di‐nucleotides (CDNs) are central signaling molecules in organisms spanning the tree of life. In bacteria, CDNs mediate many important physiological functions such as biofilm formation, motility, and virulence. CDNs are also implicated in activation of cellular biological defense systems in both bacteria and eukaryotes. In bacteria, the CDN 3’3’‐cyclic GMP‐AMP (3’3’‐cGAMP) activates a putative phage defense system in Vibrio cholerae and controls central physiological processes in Geobacter sulfurreducens and Bdellovibrio bacteriovorus. Therefore, access to a rapid, accurate 3’3’‐cGAMP quantification assay would enable further studies of this signaling molecule. Here, we describe validation of a novel 3’3’‐cGAMP enzyme linked immunoassay (ELISA) developed by Cayman Chemicals. We demonstrate that the concentrations of 3’3’‐cGAMP determined by this ELISA strongly correlate with those obtained using liquid chromatography‐tandem mass spectrometry (LC‐MS/MS). Furthermore, during these studies we show that the V. cholerae 3’3’‐cGAMP synthase, DncV, when expressed by itself in Escherichia coli, is not activated by phage infection.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.