The crosslinked peptides were identified using the xQuest/xProphet software (https ://prote omics .ethz.ch/) 51 , and the crosslinks were visualized using the webserver xVis (https ://xvis.genze ntrum .lmu.de/ login .php) 53. The mass spectrometry raw data used in this study have been deposited to the proteomeXchange Consortium via the JPOST repository (PXD019160, https ://prote omece ntral .prote omexc hange .org/cgi/GetDa taset ?ID=PXD01 9160) 54. Data accessibility The cryo-EM map of the LIN28B nucleosome has been deposited in the Electron Microscopy Data Bank, with the EMDB ID codes EMD-30070. The mass spectrometry raw data have been deposited to the proteomeXchange Consortium via the JPOST repository, with the ID code PXD019160.
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