Background: This article examines the policymaking process of Ontario's Green Energy Act (GEA) which enabled the introduction of the first comprehensive feed-in tariff (FIT) in North America. The FIT, which is a payback system for renewable energy (RE) producers, was implemented in order to make Ontario a Green Leader in North America. This article analyzes this process through a consideration of the role played by grassroots action. Methods: The Green Energy Act Alliance (GEAA) succeeded in the implementation of the GEA. Using Lober's collaborative window, this article explores factors that led to the realization of the FIT. The conclusion will show that the GEAA succeeded in opening the collaborative window, even though the model's prerequisites were not completely fulfilled, namely there was a lack of public awareness.
Long interspersed element 1 (LINE-1, or L1) is a retrotransposon that constitutes ~ 17% of the human genome. Although ~ 6000 full-length L1s spread throughout the human genome, their biological significance remains undetermined. The L1 5′ untranslated region has bidirectional promoter activity with a sense promoter driving L1 mRNA production and an antisense promoter (ASP) driving the production of L1-gene chimeric RNAs. Here, we stimulated L1 ASP activity using CRISPR-Cas9 technology to evaluate its biological impacts. Activation of the L1 ASP upregulated the expression of L1 ASP-driven ORF0 and enhanced cell growth. Furthermore, the exogenous expression of ORF0 also enhanced cell growth. These results indicate that activation of L1 ASP activity fuels cell growth at least through ORF0 expression. To our knowledge, this is the first report demonstrating the role of the L1 ASP in a biological context. Considering that L1 sequences are desilenced in various tumor cells, our results indicate that activation of the L1 ASP may be a cause of tumor growth; therefore, interfering with L1 ASP activity may be a potential strategy to suppress the growth.
Figure 1: Avatar ambidexterity allows affordance attitude alignment: As the subjective camera orbits in an "inspection gesture" between mirrored and tethered perspectives around an objective character, which is continuously animated by the connection between real-life motion capture and mixed reality environment rigging, the avatar automatically switches manipulating hand to preserve intuitive natural association.
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