Despite the high accuracy obtained through high throughput sequencing (HTS) platforms, including sequencing methods such as pyrosequencing (Roche 454), ligase (SOLiD System), and recently post-light sequencing using the Ion Torrent PGM that is able to detect ions released during sequencing, there are still many errors inherent in the chemistry used by these platforms, for example, INDEL (Insertion/Deletion) that are very common on platforms 454 of Roche and Ion Torrent PGM; Substitution abound in Illumina platforms and SOLiD. Thus, efforts to address these problems have been undertaken by the sequencing companies. To improve the accuracy of the Ion Torrent PGM reads, Life Technologies has developed an enzyme called Hi-Q.
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