The pentachlorophenol (PCP) mineralizing bacterium Sphingomonas chlorophenolica ATCC39723 degrades PCP via 2,6-dichlorohydroquinone (2,6-DCHQ). The pathway converting PCP to 2,6-DCHQ has been established previously; however, the pathway beyond 2,6-DCHQ is not clear, although it has been suggested that a PcpA plays a role in 2,6-DCHQ conversion. In this study, PcpA expressed in Escherichia coli was purified to homogeneity and shown to have novel ring-cleavage dioxygenase activity in conjunction with hydroquinone derivatives, and converting 2,6-DCHQ to 2-chloromaleylacetate.z 1999 Federation of European Biochemical Societies.
A simple and quick method for the quantitative determination of aldehydes in oxidized oils was established. The analysis was based on the reaction of N,N-dimethylp-phenylenediamine IDPPD) with aldehydes in the presence of acetic acid. Reaction products were determined by visible absorption at wavelengths of 400, 460 and 500 nm. The errors of the DPPD method in the analyses of total aldehydes in authentic mixtures of various aldehydes were less than 5%, and this method could be used successfully to determine aldehyde contents in oxidized vegetable and fish oils. The reaction time of the DPPD method 110 min at 30~ is much shorter than that of the conventional method that uses 2,4-dinitrophenylhydrazine as a reagent (30 min at 60~The simplicity of the procedure enables a quick determination of aldehyde contents in oxidized oil samples.
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