Key Points FMT was safely performed in SCT patients, with 3 complete responses and 1 partial response. Temporal microbiota dynamics seem linked to gut condition and effector regulatory T cells also increased during response to FMT.

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changes and loses its coherence, leading to a drastic reduction in the intensity of the induced excitation source.

Somatic mutations of ASXL1 are frequently detected in age-related clonal hematopoiesis (CH). However, how ASXL1 mutations drive CH remains elusive. Using knockin (KI) mice expressing a C-terminally truncated form of ASXL1-mutant (ASXL1-MT), we examined the influence of ASXL1-MT on physiological aging in hematopoietic stem cells (HSCs). HSCs expressing ASXL1-MT display competitive disadvantage after transplantation. Nevertheless, in genetic mosaic mouse model, they acquire clonal advantage during aging, recapitulating CH in humans. Mechanistically, ASXL1-MT cooperates with BAP1 to deubiquitinate and activate AKT. Overactive Akt/mTOR signaling induced by ASXL1-MT results in aberrant proliferation and dysfunction of HSCs associated with age-related accumulation of DNA damage. Treatment with an mTOR inhibitor rapamycin ameliorates aberrant expansion of the HSC compartment as well as dysregulated hematopoiesis in aged ASXL1-MT KI mice. Our findings suggest that ASXL1-MT provokes dysfunction of HSCs, whereas it confers clonal advantage on HSCs over time, leading to the development of CH.

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The description and theoretical analysis of a noise shielding system are presented. In this system, the noise and/or sound are transmitted through the piezoelectric curved membrane, which is connected to an external feedback circuit. Using the principle of elasticity control, i.e., utilization of both direct and inverse piezoelectric effects simultaneously, the movement of the membrane as well as the sound pressure of the transmitted wave can be controlled to a large extent. Transmission loss of an audible sound through the membrane in such a system is expressed as a function of a sound frequency, geometrical properties of the membrane, and parameters of the feedback circuit. It is used for the comparison of theoretical predictions and experimental data. Using this technique, the increase of the transmission loss of about 60 dB in a narrow frequency range or about 7 dB in the broad frequency range has been achieved. The performance of this system is discussed.

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In this study, the efficiency of ultrasonic disruption of Chaetoceros gracilis, Chaetoceros calcitrans, and Nannochloropsis sp. was investigated by applying ultrasonic waves of 0.02, 0.4, 1.0, 2.2, 3.3, and 4.3 MHz to algal suspensions. The results showed that reduction in the number of algae was frequency dependent and that the highest efficiency was achieved at 2.2, 3.3, and 4.3 MHz for C. gracilis, C. calcitrans, and Nannochloropsis sp., respectively. A review of the literature suggested that cavitation, rather than direct effects of ultrasonication, are required for ultrasonic algae disruption, and that chemical effects are likely not the main mechanism for algal cell disruption. The mechanical resonance frequencies estimated by a shell model, taking into account elastic properties, demonstrated that suitable disruption frequencies for each alga were associated with the cell's mechanical properties. Taken together, we consider here that physical effects of ultrasonication were responsible for algae disruption. HighlightsThe disruption of algae is frequency dependent and algae specific.The resonance frequencies of algae are calculated using elastic modulus measures.Cavitation bubbles are necessary for the algae disruption process.Chemical effects are not the main mechanism for algal cell disruption.Suitable disruption frequencies are associated with the cell's mechanical properties.