In order to study the tolerance and removal capability of Salix matsudana to phenol wastewater, we determined the effects of different concentrations of phenol on the photosynthesis, chlorophyll fluorescence parameters and enzyme activities of S. matsudana cut seedlings and their purification effects to phenol using hydroponics. The results show that wastewater containing 30 mg L-1 and 60 mg L-1 phenol increased Pn, Gs and Tr of S. matsudana, however, the maximum photochemical efficiency (Fv/Fm) of PSII changed little. When the concentration of phenol increased to 90 mg L-1-180 mg L-1, Pn and Fv/Fm of S. matsudana were significantly lower than those in the control group, while the Ci increased significantly. The 30 mgL-1-150 mgL-1 of phenol can increase the SOD and POD activities in leaves and roots of S. matsudana and the 180 mgL-1 of phenol decreased their activities. The percent removal of phenol decreased with increasing concentration of phenol ranging from 87% to 98% in 10 days. In conclusion, S. matsudana can be used to purify waste water containing phenol in concentration less than 150 mg L-1.
To explore the potential of phytoremediation 2,4-dinitrophenol (2,4-DNP) by Salix babylonica, its physiological response to 2,4-DNP, and the effect of exogenous selenium (Se) addition on its physiological response were studied in hydroponics. The results indicate that an increasing concentration of 2,4-DNP decreased the plant net photosynthetic rate (P n ), transpiration rate (T r ), chlorophyll content and increased intercellular CO 2 concentration (C i ). Both concentrations of Se increased P n and chlorophyll content and reduced C i . At low concentrations of 2,4-DNP, Se addition reduced POD and SOD activity, whereas at high concentrations its effect was reversed. In conclusion, the non-stomatal factor was the main reason for 2,4-DNP inhibiting the photosynthesis of S. babylonica. The tolerance of S. babylonica to 2,4-DNP was strong when 2,4-DNP concentration is between 10 and 20 mg . L -1 . Adding low concentration Se (≤2 μmol . L -1 ) can promote the photosynthesis of S. babylonica and enhance its tolerance to 2,4-DNP pollution.
The purpose of this paper is to explore the effects of acid treatment on root morphology and architecture in seedlings of Malus hupehensis var. pingyiensis. The rootstock seedlings were cultured in 1/2 Hoagland nutrient solutions of different pH (pH 4, pH 4.5, pH 5 and pH 6), respectively. The parameters of root architecture were measured in the day 4, 8 and 12 with the professional WinRHIZO 2007. Compared with the control (pH 6), the treatments significantly decreased the fractal dimension, length, diameter, surface area and volume of roots in day 8 and 12, and they kept decreasing followed the increase of the acidity and treatment time. The growth of lateral roots was more susceptible to acid treatment than taproots. In addition, the acid treatment mainly inhibited the growth of rootlets, significantly decreased the proportion of rootlets that changed the composition of roots, and then simplified the space structure of roots.
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