One practical approach towards robust and stable biomimetic platforms is to generate hybrid bilayers that incorporate both lipids and block co-polymer amphiphiles. The currently limited number of reports on the interaction of glass surfaces with hybrid lipid and polymer vesicles-DOPC mixed with amphiphilic poly(ethylene oxide-b-butadiene) (PEO-PBd)-describe substantially different conclusions under very similar conditions (i.e., same pH). In this study, we varied vesicle composition and solution pH in order to generate a broader picture of spontaneous hybrid lipid/polymer vesicle interactions with rigid supports. Using quartz crystal microbalance with dissipation (QCM-D), we followed the interaction of hybrid lipid-polymer vesicles with borosilicate glass as a function of pH. We found pH-dependent adsorption/fusion of hybrid vesicles that accounts for some of the contradictory results observed in previous studies. Our results show that the formation of hybrid lipid-polymer bilayers is highly pH dependent and indicate that the interaction between glass surfaces and hybrid DOPC/PEO-PBd can be tuned with pH.
Hybrid lipid/polymer membranes offer superior tunability over lipid-only systems, for a wide range of potential applications in surface modification and drug delivery, including nanovesicles (NVs) for mRNAbased therapeutics. The polymeric component can be readily modified for specific properties, and the lipid-to-polymer composition can be adjusted for even finer control. However, these materials are still an emerging field of study, and their potential is not yet fully understood. Modulating and modeling the ζ potential of these membranes is a critical step toward controlling their surface interactions. In this study, we performed ζ potential analysis on NVs with diameters of ∼100 nm made from lipids (1,2-dioleoyl-3-trimethylammonium propane, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine, and 1,2-dioleoyl-sn-glycero-3-phosphocholine) and amphiphilic block copolymers (PEO−PBd, PBd−PEO−NH 3 + , and PBd−PEO−COO − ) at various compositions. This study revealed that NVs composed of the polymer PEO−PBd are surprisingly resilient to attempts to change their ζ potential with the addition of anionic or cationic lipids, which could limit their utility for drug-delivery applications and strategies to modify their surface chemistry. To overcome these limitations and explain these results, we devised a simple predictive and explanatory model that allows estimation of the ζ potential of hybrid NVs containing charged lipids. Our results indicate that the location of the charged groups can be controlled in order to impact the visibility of those charged groups to the surrounding materials and tissues that synthetic NVs interact with. We anticipate that our approach will greatly facilitate the design and modulation of the ζ potential and other properties of polymer NVs and other biomimetic membrane materials for nanomedicine and nanobiosensors.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.