No abstract

Synovial explants and isolated synovial cells will undergo chondrogenesis when cultured in the presence of TGFbeta1. The data indicate a possible synovial origin for the chondrocytic cells found in rheumatoid pannus. Furthermore, these data are consistent with the clinical findings of synovial chondrogenesis leading to synovial chondromatosis.

Bone marrow stromal cells isolated from a model of osteoon the edges of the trabecular bone of the injected and genesis imperfecta (oim) mice, were transduced with a contralateral femurs by X-gal staining and PCR analysis at retrovirus (BAG) carrying the LacZ and neo r genes after 4, 10, 20, 30 and 40 days after injection. The LacZ gene passage 21. The transduced cells retained the ability to was also detected in the lung and liver of the recipient mice express alkaline phosphatase activity in vitro when treated at 4 and 10 days after injection but not at later time-periods. with recombinant human bone morphogenetic protein twoThe present findings suggest that long-term cultured bone (rhBMP-2), formed cartilage in vitro in aggregate cultures marrow stromal cells from osteogenesis imperfecta (OI) and formed bone in ceramic cubes after 6 weeks of implananimals have the potential to traffic through the circulatory tation in nude mice. X-gal staining of ceramic cubes system, home to bone, form bone and continue to express seeded with the transduced cells demonstrated the presexogenous genes. These findings open the possibility of ence of LacZ-positive cells on the edges of bone and also using these cells as vehicles to deliver normal genes to in the lacunae of the newly formed bone 6 weeks after bone as an alternative approach for the treatment of some implantation. After infusion into femurs of oim mice, the forms of OI and certain other bone acquired and genetic transduced cells were detected in the marrow cavity and diseases.

We examined the usefulness of neutrophil CD64 expression in detecting local musculoskeletal infection and the impact of antibiotics on its expression. Of 141 patients suspected of musculoskeletal infection, 46 were confirmed by microbiological culture to be infected and 95 had infection excluded. The median CD64 count of patients with localised infection was 2230 molecules per cell (interquartile range (IQR) 918 to 4592) and that of the patients without infection was 937 molecules per cell (IQR 648 to 1309) (p < 0.001). The level of CD64 correlated with the CRP level in patients with infection, but not in those without infection (r = 0.59, p < 0.01). Receiver operator characteristic curve analysis revealed that CD64 was a good predictor of local infection. When the patients were subdivided into two groups based on the administration of antibiotics at the time of CD64 sampling, the sensitivity for detecting infection was better in those who had not received antibiotics. These results suggest that measurement of CD64 expression is a useful marker for local musculoskeletal infection.

BackgroundNeutrophil CD64 has been reported to be a sensitive and specific infection marker. Its measurement is thus considered to be useful in early diagnosis of post-operative periprosthetic infection. However, even its normal sequential changes after non-infectious total joint arthroplasty have remained ambiguous. Accordingly, we analyzed 2-week sequential neutrophil CD64 expression changes after total joint arthroplasty in order to clarify its normal postoperative kinetics.Patients and methodFrom 41 patients who underwent primary total joint arthroplasties, peripheral blood samples were obtained at 1 day before (baseline) and 1, 3, 5, 7, and 14 days after surgery, and CD64 expression per cell was quantitatively measured. C-reactive protein (CRP) level, erythrocyte sedimentation rate (ESR) and white blood cell count (WBC) were simultaneously measured.ResultsNo cases of postoperative local infection were observed. Levels of CD64 significantly elevated from day 1, peaked at day 3, and decreased significantly following day 5. Statistical analysis confirmed that significant differences existed between the baseline level and the levels at days 1 and 3, while no significant differences existed between the baseline level and those at days 5, 7 or 14. In 17 patients, CD64 peaked at over 2,000 molecules/cell, the level reported to be a cutoff value for distinguishing infection. Multiple regression analysis showed that the sole parameter of baseline CD64 level significantly explained the peak CD64 level. Postoperative CD64 peaks ranged from 1.6 to 2.7 times (median 1.9) the baseline levels. CRP, ESR and WBC also showed rapid elevations and all but WBC remained significantly higher than baseline at day 14.ConclusionCD64 levels rise significantly, peaking within about 3 days following normal total joint arthroplasty, but decrease rapidly to near baseline within about 5 days. The data obtained can be expected to form a possible basis for early diagnosis of postoperative periprosthetic infection.