A própolis, um produto resinoso coletado por abelhas, de diferentes exsudados vegetais, é útil na manutenção e segurança da colmeia. Essa substância tem despertado o interesse de muitos pesquisadores devido às suas inúmeras propriedades terapêuticas, tais como anti-inflamatória, cicatrizante, antioxidante, antimicrobiana, anestésica, anticancerígena, dentre outras. O objetivo deste trabalho foi avaliar a ação antimicrobiana in vitro de três marcas de extrato de própolis comercializadas em Barra do Garças -MT em diferentes patógenos humanos (Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Candida albicans), bem como realizar algumas análises preconizadas pelo Ministério da Agricultura (características organolépticas, atividade antioxidante e teor de flavonoides e fenóis totais) dos mesmos extratos. Os resultados permitiram observar que as três amostras avaliadas se apresentaram adequadas quanto ao aroma, cor e sabor que caracterizam os extratos de própolis. Quanto à determinação espectrofotométrica quantitativa, o teor de flavonoides e fenóis totais apresentaramse dentro dos padrões estabelecidos pela legislação vigente. Com relação à atividade antioxidante, as médias dos valores encontrados ultrapassaram o valor exigido pelo regulamento técnico para identidade e qualidade dos extratos de própolis, demonstrando que os extratos não tinham marcante atividade redutora. A caracterização de alguns constituintes fitoquímicos nas amostras permitiu demonstrar a possível presença de flavonoides (confirmada na quantificação) e taninos condensados. Observou-se que nenhum dos extratos de própolis apresentou inibição ao crescimento dos patógenos testados. Palavras-chaves: Extrato de própolis. Atividade antimicrobiana. Flavonoides. Fenóis.
The antibacterial effect of garlic extract (5, 10 and 15%) was investigated on poultry carcasses obtained from a slaughterhouse, stored under refrigeration, and evaluated at selected time intervals. The effect of the garlic extract on the microbial contaminants of the poultry carcass surface – Salmonella, strict and facultative aerobic, mesophilic, and total and fecal coliforms – was evaluated. The garlic extract exhibited a concentration‐dependent reduction of microbial contamination. Garlic extract concentrations of 10 and 15% were the most effective. The bacteriostatic action of garlic extract against mesophilic microbiota can be observed until the third storage day. The count of total and fecal coliforms remained low during the storage period. Chicken feed was the apparent source of Salmonella contamination, and the aqueous garlic extract was not effective against Salmonella.
química, microbiológica e sensorial de queijo minas frescal de leite de cabra... Rev. Inst. Laticínios Cândido Tostes, v. 71, n. 3, p. 166-178, jul/set, 2016 que apresentou menor contagem de bactérias láticas totais e acidez, maior valor de pH e maior aceitabilidade sensorial e intenção de compra diferindo do queijo obtido por fermentação lática. Os resultados mostram que a forma de acidificação afeta as características do produto e sua aceitação pelos consumidores. A utilização do leite de cabra para produção do queijo Minas frescal apresenta potencial de aplicação na indústria de lácteos, agrega valor, tornando-o um produto alternativo aos consumidores alérgicos ao leite bovino.Palavras-chave: ácido lático; cultura starter; leite de cabra; queijo Minas frescal. ABSTRACTThe aim of this study was to evaluate the effect of the direct acidification by lactic acid and indirect, by adding of the mesophilic starter culture type O (Lactococcus lactis subsp. lactis e Lactococcus lactis subsp. cremoris), the physicochemical properties, microbiological and sensory product. Two formulations of Minas Frescal cheese were prepared by acidification with lactic acid and acidification with mesophilic type O culture and evaluated for microbiological quality: the psychrotrophic count, aerobic mesophilic and thermotolerant Coliforms and Salmonella sp. The physicochemical analyzes were performed determination of fat, ash, moisture, acidity in lactic acid and pH. The physical and chemical changes were evaluated in the Minas fresh cheese during the 14-day storage period, and analyzed its acceptability and purchase intent through sensory analysis. The results revealed that the Minas fresh cheese formulations presented in good sanitary conditions and of consumption. The physico-chemical parameters of fat, ash and moisture of the Minas fresh cheeses do not differ significantly (p < 0.05). The cheese obtained by direct acidification by lactic acid showed the lowest values of count total lactic bacteria and acidity in acid lactic, higher pH and greater sensory acceptance and purchase intent differing cheese obtained by lactic fermentation. The results show that the form of acidification affects the product characteristics and consumer acceptability. The use of goat milk for the production of fresh cheese mines shows potential for the dairy industry and adds value to the product and becomes an alternative product to consumers allergic to cow's milk.
The use of the same antibiotics in animal food and conventional medicine can cause problems in the treatment of human diseases, because poultry frequently carry human pathogens. The aim of the present study was to determine the antibiotic resistances patterns of Campylobacter jejuni isolated from the rearing environment of broiler chickens – litter, drinking water, feed and the bird's cloaca. The results showed significant resistance to the following antibiotics: sulfonamide (77%), tetracycline (100%), erythromycin (80%) and penicillin G (100%). Intermediary resistance was present in bacitracin (90%), trimethoprim (100%), vancomycin (100%), chloramphenicol (97%), nalidixic acid (100%) and azithromycin (100%). However, new studies need to be carried out in Brazil to determine the resistance amplitude of this microorganism in other animals and humans. It is important to define some control strategies of the antibiotics used in animal production and human medicine.
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