H 2 O 2 permeation through a cell membrane significantly affects living organisms, and permeation is controlled by the physico-chemical nature of lipids and other membrane components. We investigated the molecular relationship between H 2 O 2 permeation and lipid membrane structure using three oxidized lipids. POVPC and PazePC act as intra- and inter-molecular permeation promoters, respectively; however, their underlying mechanisms were different. The former changed the partition equilibrium, while the latter changed the permeation pathway. PoxnoPC inhibited permeation under our experimental conditions via an intra-molecular configuration change. Thus, both intra- and inter-molecular processes were found to control the role of oxidized lipids as inhibitors and promoters towards H 2 O 2 permeation with different mechanisms depending on structure and composition. Here, we identified two independent H 2 O 2 permeation routes: (i) permeation through lipid membrane with increased partition coefficient by intra-molecular configurational change and (ii) diffusion through pores (water channels) formed by inter-molecular configurational change of oxidized lipids. We provide new insight into how biological cells control permeation of molecules through intra- and inter-molecular configurational changes in the lipid membrane. Thus, by employing a rational design for both oxidized lipids and other components, the permeation behaviour of H 2 O 2 and other ions and molecules through a lipid membrane could be controlled.
Nanomaterials has recently been expected as one of promising materials for functional anti-microbial agent, owing to their high activity high stability and low-cost fabrication process. Here, we have clarified the mechanism of destructive interaction of polyoxometalate (POM) clusters with the model cell membrane, by using both liposomes and solidsupported lipid bilayers. Leakage experiment clarified that liposomes are broken by POM cluster above a critical threshold concentration. At these concentrations, a formation of network-like defect in the solid-supported bilayer was found by fluorescence microscope observations. From correlations between results of liposome and supported bilayers, it was concluded that the formation of network-like defects formed above threshold concentration is the origin of destructive activity of POM for the cell membranes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.