This study tests the hypothesis that invasion of partially transformed keratinocytes is initiated by diffusible, proinvasive signals provided by species-specific fibroblasts. In vitro organotypic cultures of neoplastic human oral mucosa were constructed by growing a partially transformed, nontumorigenic keratinocytic cell line isolated from a dysplastic human oral lesion (DOK-ECACC94122104) on top of various types of connective tissue equivalents. Cultured tissues were analyzed by histomorphometry (depth and area of invasion: D inv , A inv ) and immunohistochemistry. Presence of human fibroblasts in the matrix induced a local invasion of DOK (D inv ؍ 95.6 ؎ 7.1 m, A inv ؍ 45.8 ؎ 3.5%). Minimal invasion (P < 0.05) was observed when DOK grew on simple collagen matrix (D inv ؍ 14.1 ؎ 2.1 m, A inv ؍ 3.7 ؎ 0.8%) or matrices containing fibroblasts from mouse (D inv ؍ 11.5 ؎ 4.0 m, A inv ؍ 4.3 ؎ 1.0%) or rat (D inv ؍ 15.6 ؎ 1.2 m, A inv ؍ 6.1 ؎ 0.5%). In these cultures, local invasion could be induced by the presence of human fibroblasts in a bottom layer of the collagen matrix (P < 0.05) or by conditioned medium from organotypic cultures of DOK on human fibroblast-containing matrix (P < 0.05) but not by conditioned medium from human fibroblast monocultures (P > 0.05). Deposition of human collagen IV was observed at epithelialmatrix interface only when DOK behaved invasively. In conclusion, invasion of partially transformed oral keratinocytes was triggered by keratinocyte-induced fibroblast-derived diffusible factor(s) in a species-specific manner and associated with de novo synthesis of collagen
<p>MP4 file - 752K, 1. Time-lapse microscopy of CAF1 plated onto collagen I showing the diversity of CAF movement, with a predominance of motile cells with longer tracks. Images were captured every 10 mins and analysed using IMARIS software. The centre of each CAF is marked by a grey square. Migration was visualized by a track that changes colour in time as shown by the scale lower right corner. Examples of highly motile CAF are shown in the left quadrants and less motile CAF at the junction of the upper and lower left quadrants. Quantification of the migration is shown in supplementary Table ST1.</p>
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