The karyotypes were normal without ring chromosome 15, and motile sperm with a high aspect ratio showed adequate potential for fertilization.

Purpose: The mechanical energy of sperm is an optimal index with which to quantitatively assess the motility of sperm. The sperm energy index (SEI) is proposed as an index representing the total mechanical energy of sperm in a single field when measured using computer-assisted sperm analysis (CASA). CASA systems can automatically view multiple fields in a specimen chamber to capture the images of sperm from a microscope, are capable of measuring sperm motility and kinematics using a computer. The mechanical energy of a single sperm is given by (SEI/motile sperm count) × 100 which has been designated S-SEI. The aim of the current study was to examine the relationship between SEI or S-SEI and fertilization rate in conventional IVF (cIVF). Methods:The materials used were 904 ova from 101 couples who underwent cIVF. SEI were measured in a suspension of sperm prior to insemination.Results: Couples were classified into 4 groups based on a median SEI of 4.1 and a median S-SEI of 4.7. Couples in group D (SEI < 4.1 and S-SEI >= 4.7) had the highest rate of normal fertilization 73.9% and the lowest rate of polyspermic fertilization. Couples in group B (SEI >= 4.1 and S-SEI >= 4.7) had the highest rate of polyspermic fertilization. Conclusions:A combination of the SEI and the S-SEI can serve as a criterion with which to determine whether couples should continue to undergo cIVF.

The developmental potential of 1PN zygotes with large pronuclear areas (≥ 710 μm) or diameters (31 μm) appears to be similar to that of 2PN zygotes, and measurement of pronuclear area or diameter in 1PN zygotes is a simple, potentially useful, clinical method.

Thermally activated delayed fluorometry was applied to the analysis of chlorophyll c and pheophytin c of phytoplankton in natural water. Acetone extract of pigments was chromatographed over paper, and chlorophyll c on the chromatogram could be directly