It is known that particle size obtained from Brownian motion of a particle is called the diffusion coefficient equivalent size (DCES) and is close to the geometric size independent of the physical properties of a particle. In this paper, it is described that real time measurement of the particle size and particle number concentration by observing the Brownian motion of the particles from which the influence of the flow field has been eliminated by the instrument combining the flow particle tracking (FPT) method and the L-shaped flow cell. Furthermore, we realized in evaluating the refractive index, which is the physical property information of the particles, from the DCES, the Rayleigh scattering equation and the light scattering intensity of particle that physical properties are known. In this paper, we also discuss that improvement of particle size accuracy and refractive index accuracy in the measurement of DCES. Index Terms-Brownian motion, diffusion coefficient equivalent size (DCES), flow particle tracking (FPT) method, physical properties of a particle, refractive index.
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