For the biological control of diamondback moth (DBM) larvae in commercial greenhouses, we have previously identified a blend of volatiles that attracted Cotesia vestalis, a parasitoid of DBM larvae. Here, we tested the effects of an artificial volatile blend on the attractiveness of komatsuna plants (Japanese mustard spinach; Brassica rapa var. perviridis) to C. vestalis under greenhouse conditions. First, we showed that female C. vestalis preferred infested komatsuna plants to uninfested plants in the greenhouse. Under the same conditions, placing the artificial attractants near both infested and uninfested plants did not affect the wasps' preference. However, when comparing infested komatsuna plants coupled with the artificial attractants with infested plants without them, significantly more female C. vestalis were attracted to the former. The possible use of artificial C. vestalis attractants for the biological control of DBM is discussed.
A blend of four volatile compounds, (Z)‐3‐hexene‐1‐yl acetate, α‐pinene, sabinene and n‐heptanal, emitted from cabbage plants infested by diamondback moth (DBM) larvae (hereafter called the attractant) attract females of Cotesia vestalis, a dominant parasitoid wasp species of DBM larvae. Here, we tested the effects of the presence of uninfested komatsuna (Brassica rapa var. perviridis L. cv. Rakuten) plants on the wasps’ response to the attractant in the choice chamber under laboratory conditions. Satiated female wasps were equally distributed between groups of 16 and four uninfested plants. However, the wasps preferred the group of 16 uninfested plants with the attractant to that of four uninfested plants with the attractant, indicating that the greater biomass of uninfested plants synergistically increased the attractiveness of the attractant. The effects of honey (a food of C. vestalis while rearing) on the wasps’ responses to the attractant were also tested using starved females. The wasps showed an equal distribution between honey with four uninfested plants and four uninfested plants. When both odour sources were coupled with the attractant, the wasps significantly preferred the former odour source, indicating a synergistic effect of honey on the attractiveness of the attractant. This effect, however, was observed when C. vestalis females had been reared with the tested honey but not when they had been reared with aqueous sucrose. In the experimental glasshouse, when comparing a group of infested plants coupled with uninfested plants, honey and the attractant to a group of infested plants, female wasps preferred the former. In addition, this preference was significantly greater than their preference for infested plants coupled with the attractant over infested plants. Thus, there was a synergistic effect of uninfested plants and honey on the attractiveness of the attractant–infested plant complex under glasshouse conditions.
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