Summary Cytokeratin (CK) 18 M30 antigen has been proposed as a diagnostic marker of nonalcoholic fatty liver disease (NAFLD). We studied serum CK18 M30 antigen level and examined the correlations among CK18 and biological data, dietary intake, and plasma fatty acid composition in middle-aged Japanese males with (NAFLD; n ϭ 42) and without NAFLD (control; n ϭ 35). NAFLD was diagnosed if subjects showed fatty liver on abdominal ultrasonography and their alcohol consumption was Ͻ 20 g/d. They were also confirmed to have negative serological results for tests of autoimmune liver disease and hepatitis B and C. In the NAFLD group, body mass index, waist circumference, serum M30 antigen, alanine transaminase (ALT), cholinesterase, triacylglycerol, LDL-cholesterol, and HbA1c were significantly higher than in the control group. In the fatty acid analysis of plasma phospholipids, significantly higher dihomo-␥ -linolenic acid (DGLA), total saturated fatty acids (SFA), and palmitic/linoleic acid ratio as well as lower arachidonic acid/DGLA ratio were observed in the NAFLD group compared with the control group. In the NAFLD group, M30 antigen was correlated positively with serum ALT, plasma DGLA, dietary SFA, and serum TNF-␣ as determined by partial correlation analysis controlled for BMI. On the basis of multivariate regression analysis using a stepwise method, M30 antigen was significantly associated with ALT and plasma DGLA. Regarding the determinants of NAFLD as revealed by logistic regression analysis, a high odds ratio was observed for plasma DGLA. In conclusion, members of the NAFLD group showed higher levels of serum CK18 M30 antigen and M30 antigen was strongly associated with serum ALT and plasma DGLA. Abnormal fatty acid metabolism may be a factor that causes aggravation of NAFLD.
We evaluated the utility of an immunocytochemical technique employing the commercially available anti‐CD56 monoclonal antibody, NKH 1. The utility and sensitivity of this technique in the detection of invasive neuroblastoma (NB) cells in the bone marrow were compared with those of Wright‐Giemsa staining. The correlation coefficient for the percent NB cells detected using Wright‐Giemsa staining with the percent NKH 1 immunoreactive cells was 0.78. In the analysis of specificity, this monoclonal antibody showed slight cross‐reactivity with normal bone marrow cells, including macrophages, lymphocytes and osteoblasts. In the evaluation of the sensitivity of the NKH 1 immunocytochemical technique, SK‐N‐DZ and SK‐N‐SH NB cell lines were added to morphologically normal bone marrow mononuclear cells from patients without NB to the final NB cell line at concentrations of 2%, 1% and 0.1%. NB cells at the final concentration of 0.1 % could be detected by the immunocytochemical technique. We conclude that the NKH 1 immunocytochemical staining technique is useful in the detection of metastatic NB cells in bone marrow.
測 定 項 目
2.1Inner Scan BC-AST ALT C : NU-CHEK-PREP, USA t-
γ-γGT
Mean SDTotal C : trans isomers Total C : trans/cis The clinical aspects of non-alcoholic fatty liver disease. Minerva Gastroenterol Dietol 54:
Summary:Trans-fatty acid TFA has been recognized as a risk factor for diseases such as dyslipidemia and cardiovascular disease. However, the effects of serum TFA levels on non-alcoholic fatty liver disease NAFLD have not been fully elucidated. In this study, we analyzed the TFA composition of plasma phospholipids in Japanese men to examine the relationship between TFA and NAFLD. In the NAFLD group, the plasma levels of linoelaidic acid 9t,12t-C : were significantly higher than in the control group, while significantly lower index levels of delta desaturase D D , which catalyzes the conversion of dihomo-γ-linolenic acid to arachidonic acid, were observed. The level of linoelaidic acid showed a significant positive correlation with the serum levels of cytokeratin CK M antigen, a parameter of apoptosis, and M antigen and alanine aminotransferase ALT , which are parameters of liver cell necrosis. Furthermore, linoelaidic acid was negatively correlated with the D D index. These results suggest that increases in the plasma phospholipid TFA levels, especially linoelaidic acid, reflect changes in lipid metabolism followed by hepatic necrosis and apoptosis in NAFLD.
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