Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens that cause illnesses in humans ranging from mild to hemorrhagic enteritis with complications of hemolytic uremic syndrome and even death. Cattle are a major reservoir of STEC, which reside in the hindgut and are shed in the feces, a major source of food and water contaminations. Seven serogroups, O26, O45, O103, O111, O121, O145 and O157, called ‘top-7’, are responsible for the majority of human STEC infections in North America. Additionally, 151 serogroups of E. coli are known to carry Shiga toxin genes (stx). Not much is known about fecal shedding and prevalence and virulence potential of STEC other than the top-7. Our primary objectives were to identify serogroups of STEC strains, other than the top-7, isolated from cattle feces and subtype stx genes to assess their virulence potential. Additional objective was to develop and validate a novel multiplex PCR assay to detect and determine prevalence of six serogroups, O2, O74, O109, O131, O168, and O171, in cattle feces. A total of 351 strains, positive for stx gene and negative for the top-7 serogroups, isolated from feedlot cattle feces were used in the study. Of the 351 strains, 291 belonged to 16 serogroups and 60 could not be serogrouped. Among the 351 strains, 63 (17.9%) carried stx1 gene and 300 (82.1%) carried stx2, including 12 strains positive for both. The majority of the stx1 and stx2 were of stx1a (47/63; 74.6%) and stx2a subtypes (234/300; 78%), respectively, which are often associated with human infections. A novel multiplex PCR assay developed and validated to detect six serogroups, O2, O74, O109, O131, O168, and O171, which accounted for 86.9% of the STEC strains identified, was utilized to determine their prevalence in fecal samples (n = 576) collected from a commercial feedlot. Four serogroups, O2, O109, O168, and O171 were identified as the dominant serogroups prevalent in cattle feces. In conclusion, cattle shed in the feces a number of STEC serogroups, other than the top-7, and the majority of the strains isolated possessed stx2, particularly of the subtype 2a, suggesting their potential risk to cause human infections.

Studies suggest a link between added copper (Cu) and co-selection of antimicrobial resistance (AMR) in Enterococcus spp., but data are inconsistent. This study aimed to assess the impact of added Cu, alone or with a feed-grade antimicrobial, on growth performance, transferable Cu resistance gene (tcrB) prevalence, abundance of tcrB in fecal community DNA, and AMR in fecal enterococci in weaned piglets. A total of 320 barrows (DNA 200 × 400, DNA Genetics) weaned at approximately 21 d of age with 7.4 kg (7.4 ± 0.06 kg) BW were used in a 28-d study. Piglets were fed a common non-medicated diet for 7 d of acclimation. Treatments were arranged in a 2 × 2 factorial design with main effects of added Cu (0 vs. 200 mg/kg Cu from Cu sulfate) and chlortetracycline (0 vs. 440 mg/kg CTC). Growth performance and fecal samples were obtained on days 0, 14, and 28. There was no evidence (P > 0.05) for Cu and CTC interaction in growth performance. Pigs fed diets with added Cu had increased (P < 0.05) ADG and ADFI from days 0 to 14, with no evidence for differences (P > 0.05) from days 15 to 28 and 0 to 28. Pigs fed diets with CTC had improved (P < 0.01) ADG, ADFI, and G:F from days 0 to 28. Prevalence of tcrB-positive enterococci was not affected by the addition of Cu and/or CTC (P > 0.05). Prevalence of tcrB-positive enterococci was higher on day 14 than other sampling days (P = 0.002). Prevalence of tetracycline resistance gene [tet(M)]-positive enterococci was not affected by treatments or day (P > 0.05). Prevalence of macrolide resistance gene [erm(B)]-positive enterococci had a significant treatment and sampling day interaction (P = 0.021). The abundance of the tcrB gene in feces, quantified by PCR, was not affected by Cu treatment. The median Cu minimum inhibitory concentrations (MIC) of tcrB-negative and -positive isolates were 3 and 20 mM, respectively (P < 0.001). For day 0 and day 28, all Enterococcus isolates were susceptible to gentamicin, kanamycin, streptomycin, daptomycin, and tigecycline, with a majority of isolates resistant to chloramphenicol, erythromycin, lincomycin, linezolid, tetracycline, tylosin tartrate, and Synercid. In conclusion, 200 mg/kg added Cu or 440 mg/kg CTC in nursery diets improved growth performance of nursery pigs. Added Cu, with or without a selection pressure of CTC, did not increase Cu-resistant enterococci and did not co-select resistance to antibiotics.

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Antibiotics can be administered via various routes in pigs, which may influence antimicrobial resistance development. A total of 40 barrows and 40 gilts (Line 600 × 241; DNA, Columbus, NE; initially 207 ± 7.9 lb) were used in a 35-d trial to determine the effects of tylosin administration route on pig growth performance and development of antimicrobial resistance in fecal Enterococcus spp. isolates. Pens of pigs (1 pig/ pen, 20 pigs/treatment) were blocked by initial body weight (BW) and gender. Within blocks, pens were randomly allotted to 1 of 4 treatments. The antibiotic treatments followed US label directions and were: 1) no antibiotic (Control); 2) 110 mg tylosin per kg of feed for 21 d (Feed); 3) 8.82 mg tylosin per kg of BW through intramuscular injection twice daily for the first 3 d of each wk during the 3-wk treatment period (Injection); and 4) 66 mg of tylosin per liter of drinking water for the first 3 d of each wk during treatment period (Water). Treatments were offered during d 0 to 21, after which all pigs were fed a common diet with no antibiotic until d 35. Fecal samples were collected on d 0, 21, and 35. No evidence for route × gender interactions (P > 0.55) were observed for any growth responses. From d 0 to 21, control pigs and pigs fed medicated feed had greater (P < 0.05) average daily gain (ADG) than those that received injected tylosin, with the ADG of pigs receiving tylosin through the water intermediate. There was no evidence for different average daily feed intake (ADFI) among treatment groups. Pigs that received tylosin through injection or water had poorer (P < 0.05) feed efficiency (F/G) compared with control pigs, but there was no evidence for difference from pigs receiving tylosin through feed. Among the medicated pigs, total tylosin dose administered was the greatest through injection, second highest through feed, with the water medication route the lowest. No evidence for route × day interactions (P > 0.23) were observed for the development of bacterial resistance to any antibiotics. Enterococcal isolates collected from pigs receiving tylosin via feed or injection were more resistant (P < 0.05) to erythromycin and tylosin compared with control pigs and those that received tylosin through water. The estimated probability of antimicrobial resistance to these 2 antibiotics was greater on d 21 and 35 than d 0. In summary, tylosin injection resulted in poorer ADG and F/G of finishing pigs, likely due to stress associated with handling and injection. Tylosin administration through injection and feed resulted in greater probability of enterococcal resistance to erythromycin and tylosin compared with in-water treatment, which is likely a combined effect of administration route and dosage.2 Swine Day 2018 (P < 0.05) to erythromycin and tylosin compared with control pigs and those that received tylosin through water. The estimated probability of antimicrobial resistance to these 2 antibiotics was greater on d 21 and 35 than d 0. In summary, tylosin injection resulted in poorer ADG and F/G ...

A total of 320 nursery pigs (DNA 200 × 400 barrows; initially 16.3 lb BW) were used in a 28-d trial to determine the effect of copper (Cu) and chlortetracycline (CTC), fed alone or in combination, on growth performance of weanling pigs. Pigs were weaned at approximately 21 d of age and fed a common pelleted starter diet (non-medicated) for 7 d after weaning. Pigs were allotted to dietary treatments based on BW and location in a randomized complete block design. Dietary treatments were arranged as a 2 × 2 factorial with main effects of added Cu (0 vs. 200 ppm Cu from copper sulfate) and CTC (0 vs. 440 ppm CTC). Experimental diets were corn-soybean meal-based and were fed in one phase for 28 d in meal form. There were 5 pigs per pen and 8 replications per treatment with each replication consisting of a pair of adjoining pens. The results showed no evidence for an interactive effect of Cu and CTC for any of the performance and economic variables (P > 0.05). From d 0 to 14, added Cu increased (P < 0.05) ADG and ADFI and added CTC improved (P < 0.05) ADG, ADFI, and F/G. From d 14 to 28, the addition of CTC to the diet improved (P < 0.05) ADG and ADFI, but there was no evidence for a Cu effect. For the overall experimental period (d 0 to 28), pigs fed diets with CTC had improved (P < 0.05) ADG, ADFI, and F/G, but there was no evidence for a Cu effect. The inclusion of either Cu or CTC increased (P < 0.05) BW on d 14 and 28. Regarding the economics, added dietary Cu increased (P < 0.05) feed cost per pig and value of gain per pig, but not income over feed cost (IOFC). The addition of CTC to the diet increased (P < 0.05) feed cost per pig, value of gain per pig, and IOFC. In conclusion, the findings of the present study characterize a beneficial effect of feeding Cu for 14 d on growth performance of young pigs (16 to 25 lb) and a positive effect of including CTC in nursery diets. The lack of interactive effects between Cu and CTC suggests that the responses of Cu and CTC on growth performance of nursery pigs are as efficacious when fed alone or in combination.

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A total of 320 nursery pigs (DNA 200 × 400 barrows; initially 16.3 lb BW) were used in a 28-d trial to determine the effect of copper (Cu) and chlortetracycline (CTC), fed alone or in combination, on growth performance of weanling pigs. Pigs were weaned at approximately 21 d of age and fed a common pelleted starter diet (non-medicated) for 7 d after weaning. Pigs were allotted to dietary treatments based on BW and location in a randomized complete block design. Dietary treatments were arranged as a 2 × 2 factorial with main effects of added Cu (0 vs. 200 ppm Cu from copper sulfate) and CTC (0 vs. 440 ppm CTC). Experimental diets were corn-soybean meal-based and were fed in one phase for 28 d in meal form. There were 5 pigs per pen and 8 replications per treatment with each replication consisting of a pair of adjoining pens. The results showed no evidence for an interactive effect of Cu and CTC for any of the performance and economic variables (P > 0.05). From d 0 to 14, added Cu increased (P < 0.05) ADG and ADFI and added CTC improved (P < 0.05) ADG, ADFI, and F/G. From d 14 to 28, the addition of CTC to the diet improved (P < 0.05) ADG and ADFI, but there was no evidence for a Cu effect. For the overall experimental period (d 0 to 28), pigs fed diets with CTC had improved (P < 0.05) ADG, ADFI, and F/G, but there was no evidence for a Cu effect. The inclusion of either Cu or CTC increased (P < 0.05) BW on d 14 and 28. Regarding the economics, added dietary Cu increased (P < 0.05) feed cost per pig and value of gain per pig, but not income over feed cost (IOFC). The addition of CTC to the diet increased (P < 0.05) feed cost per pig, value of gain per pig, and IOFC. In conclusion, the findings of the present study characterize a beneficial effect of feeding Cu for 14 d on growth performance of young pigs (16 to 25 lb) and a positive effect of including CTC in nursery diets. The lack of interactive effects between Cu and CTC suggests that the responses of Cu and CTC on growth performance of nursery pigs are as efficacious when fed alone or in combination. KeywordsCu, CTC, feed-grade antibiotic, supplementation, weanling pig Creative Commons LicenseThis work is licensed under a Creative Commons Attribution 4.0 License. Cover Page FootnoteAppreciation is expressed to Micronutrients (Indianapolis, IN) for financial support. http://newprairiepress.org/kaesrr/vol3/iss7/28 Swine Day 2017 K A N S A S STAT E UNI VE R S IT Y 2017Kansas State University Agricultural Experiment Station and Cooperative Extension Service SummaryA total of 320 nursery pigs (DNA 200 × 400 barrows; initially 16.3 lb BW) were used in a 28-d trial to determine the effect of copper (Cu) and chlortetracycline (CTC), fed alone or in combination, on growth performance of weanling pigs. Pigs were weaned at approximately 21 d of age and fed a common pelleted starter diet (non-medicated) for 7 d after weaning. Pigs were allotted to dietary treatments based on BW and location in a randomized complete block design. Dietary treatments were arranged a...