Social insects are ecologically dominant and provide vital ecosystem services. It is critical to understand collective responses of social insects such as bees to ecological perturbations. However, studying behavior of individual insects across entire colonies and across timescales relevant for colony performance (i.e., days or weeks) remains a central challenge. Here, we describe an approach for long-term monitoring of individuals within multiple bumble bee (Bombus spp.) colonies that combines the complementary strengths of multiple existing methods. Specifically, we combine (a) automated monitoring, (b) fiducial tag tracking, and (c) pose estimation to quantify behavior across multiple colonies over a 48 h period. Finally, we demonstrate the benefits of this approach by quantifying an important but subtle behavior (antennal activity) in bumble bee colonies, and how this behavior is impacted by a common environmental stressor (a neonicotinoid pesticide).
Social bees are critical for supporting biodiversity, ecosystem function and crop yields globally. Colony size is a key ecological trait predicted to drive sensitivity to environmental stressors and may be especially important for species with annual cycles of sociality, such as bumblebees. However, there is limited empirical evidence assessing the effect of colony size on sensitivity to environmental stressors or the mechanisms underlying these effects. Here, we examine the relationship between colony size and sensitivity to environmental stressors in bumblebees. We exposed colonies at different developmental stages briefly (2 days) to a common neonicotinoid (imidacloprid) and cold stress, while quantifying behaviour of individuals. Combined imidacloprid and cold exposure had stronger effects on both thermoregulatory behaviour and long-term colony growth in small colonies. We find that imidacloprid's effects on behaviour are mediated by body temperature and spatial location within the nest, suggesting that social thermoregulation provides a buffering effect in large colonies. Finally, we demonstrate qualitatively similar effects in size-manipulated microcolonies, suggesting that group size per se , rather than colony age, drives these patterns. Our results provide evidence that colony size is critical in driving sensitivity to stressors and may help elucidate mechanisms underlying the complex and context-specific impacts of pesticide exposure.
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