We report the redox mediation of glucose oxidase (GOx) in a
self-assembled structure of cationic poly(allylamine) modified by ferrocene (PAA-Fc) and anionic GOx deposited
electrostatically layer-by-layer on
negatively charged alkanethiol-modified Au surfaces. Successive
PAA-Fc and GOx layers were deposited
by alternate immersion of the thiol-modified Au in the respective
polyelectrolyte and enzyme solutions.
The uptake of thiol, redox polymer, and GOx on the surface was
monitored by quartz crystal microbalance.
Cyclic voltammetry shows nearly ideal surface waves of ferrocene
in the polymer with charge independent
of sweep rate; the redox surface concentration was obtained from
integration of the ferrocene/ferricinium
voltammetric peaks. The redox charge increases in step with the
number of PAA-Fc layers deposited.
Enzyme catalysis for the oxidation of β-d-glucose
was achieved with a multilayer PAA-Fc/GOx assembly,
with each GOx layer contributing equally to the catalytic response.
Only a small fraction of the active
assembled GOx molecules are “electrically wired” by the ferrocene
polymer although all of the enzyme
could be oxidized by soluble ferrocenesulfonate when added to
solution.
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Imidazole derivatives R 0190Environmentally Benign Approach to Imidazole-2-thiones. -A microwave-promoted synthesis of substituted imidazolethiones (III) (12 examples) from benzoin (I) and substituted thioureas (II) is achieved under solvent-free conditions . -(KIDWAI*, M.; KUKREJA, S.; RASTOGI, S.; SINGHAL, K.; Indian J. Chem., Sect.
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