Serial section microscopy has proven to be a powerful tool in examining the 3-dimensional (3D) nature of tissue; however, it is limited by the size of tissue samples that can be used and the manual process of acquiring data. The knife-edge scanning microscope (KESM) acquisition and analysis computational system resolves these issues by providing high-resolution light microscopy image data at multi-cubic centimeter scale. Using this system for quantification of microvasculature provides the ability to examine, measure, and compare the 3D nature of vascular networks at whole organ scale and sub-micron resolution.
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