Chitosan (QT) is a biopolymer that has been used in widely used in bone tissue engineering applications, demonstrating great potential for that purpose. Therefore, the present study aims to develop an in situ crosslinking hydrogel system, composed of chitosan and hydroxyapatite (HAP). Briefly, chitosan was modified, using carbodiimide chemistry, with lactobionic and azidobenzoic acid to make it soluble at physiological pH and photo-crosslinkable, respectively. The modified chitosan was mixed with HAP, in different proportions, and later exposed to UV light, yielding hydrogels. Mesenchymal stem cells, from rat bone marrow, were seeded onto the hydrogels and cultured for 4, 10 and 16 days, under osteogenic and non-osteogenic conditions. Through cell proliferation and alkaline phosphatase activity assays, and confocal microscopy, it was observed that the majority of formulations supported cell adhesion and proliferation, and a significant early osteogenic differentiation in formulations 70:30 and 50:50, QT:HAP. According to these results, the proposed photo-crosslinking system has potential for tissue engineering applications, and further specific studies are proposed for cell differentiation.
O objetivo deste trabalho é desenvolver pesquisa bibliográfica sobre o castelhano boliviano, contrastando as variedades colla e camba presentes na fronteira Puerto Quijarro (Bolívia) e Corumbá (Brasil). Trata-se de um estudo linguístico que se inicia com uma breve história das variedades do castelhano boliviano, nos aspectos fonético e morfológico, as mesmas que estão invisibilizadas no âmbito educativo fronteiriço mencionado, embora estejam presentes no falar dos alunos de origem boliviana que frequentam as escolas brasileiras. Callisaya Apaza (2012), Coello Vila (1996), e Mendoza Quiroga (2015) balizam os estudos sobre o castelhano boliviano. Franco e Gottret (2022), Sanabria Fernández (2008) e Roca (2007) contribuem com os estudos sobre o castelhano colla e camba. Esse último encontra-se materializado no ramo publicitário e nas redes sociais, entre outros meios que, por vezes, utilizam a linguagem coloquial na comunicação com o público alvo. Neste trabalho são expostos exemplos veiculados em uma rede social.
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