Previously isolated DNA damage-inducible (din) genes of Bacillus sublilis have been mapped on the bacterial chromosome by bacteriophage PBS1-mediated transduction. The din genes have been localized to three positions on the B. subtilis map. dinA cotransduction with the hisA locus was 80%, while dinC cotransduction with this marker was about 56%. dinB is unlinked to hisA, but its cotransduction with the dal-1 and purB loci was 84 and 22%, respectively.
The mechanical behavior of lnconel 718 processed using the Delta Process (DP) conversion technique was studied. The DP conversion sequence uses a intentional delta phase precipitation cycle and subsequent thermomechanical processing to produce uniform fine grain billet and bar stock. The process was developed to improve the mechanical properties through the elimination of occasional coarse grains which are common in conventionally processed materials. The net effect resulted in a decreased standard deviation in the mechanical properties and an increase in the minimum properties. The microstructure, tensile, low cycle fatigue, and cyclic crack growth behaviors of DP718 impeller, disk forgings and bar stock were investigated. Forged DP718 properties were compared to conventionally processed lnconel 718 and another high strength material. The DP718 bar stock properties were compared to conventionally processed lnconel 718.
Flanking recombination events have defined the Huntington Disease gene candidate region to between D4S10 and D4S98, about 2.2Mb. Because of the large size of the candidate region and the likely large number of genes within it we decided to screen cDNA libraries with probes generated from whole Yeast Artificial Chromosomes (YACs) containing parts of this region. We have thus far used 4 YACs ranging in size from 180kb to 600kb covering 880kb and have isolate 13 cDNA clones, 7 of which are unique. Three of the 13 clones contain parts of the 3' untranslated region of the alpha-adducin gene. One YAC of 600kb could not be purified from two yeast chromosomes, therefore this YAC probe had a net complexity of 1.8Mb. Even so this probe identified a cDNA from the HD candidate region indicating that very large YACs may be used as probes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.