The mammalian endoglycosidase heparanase (Hpa1) is primarily responsible for cleaving heparan sulphate proteoglycans (HSPGs) present on the basement membrane of cells and its potential for remodelling the extracellular matrix (ECM) could be important in embryonic development and tumour metastasis. Elevated expression of this enzyme has been implicated in various pathological processes including tumour cell proliferation, metastasis, inflammation and angiogenesis. The enzyme therefore represents a potential therapeutic target. Hpa1 protein is initially synthesized as an inactive 65 kDa proenzyme that is then believed to be subsequently activated by proteolytic cleavage to generate an active heterodimer of 8 and 50 kDa polypeptides. By analysis of a series of Hpa1 deletion proteins we confirm that the 8 kDa subunit is essential for enzyme activity. We present here for the first time an insect cell expression system used for the generation of large amounts of recombinant protein of high specific activity. Individual subunits were cloned into baculoviral secretory vectors and co-expressed in insect cells. Active secreted heterodimer protein was recovered from the medium and isolated by a one-step heparin-Sepharose chromatography procedure to give protein of >90% purity. The recombinant enzyme behaved similarly to the native protein with respect to the size of HS fragments liberated on digestion, substrate cleavage specificity and its preference for acidic pH. A significant amount of activity, however, was also detectable at physiological pH values, as measured both by an in vitro assay and by in vivo degradation of cell-bound heparan sulphate.

The upper-air sounding network for Dynamics of the Madden-Julian Oscillation (DYNAMO) has provided an unprecedented set of observations for studying the MJO over the Indian Ocean, where coupling of this oscillation with deep convection first occurs. With 72 rawinsonde sites and dropsonde data from 13 aircraft missions, the sounding network covers the tropics from eastern Africa to the western Pacific. In total nearly 26 000 soundings were collected from this network during the experiment's 6-month extended observing period (from October 2011 to March 2012). Slightly more than half of the soundings, collected from 33 sites, are at high vertical resolution. Rigorous post-field phase processing of the sonde data included several levels of quality checks and a variety of corrections that address a number of issues (e.g., daytime dry bias, baseline surface data errors, ship deck heating effects, and artificial dry spikes in slow-ascent soundings).Because of the importance of an accurate description of the moisture field in meeting the scientific goals of the experiment, particular attention is given to humidity correction and its validation. The humidity corrections, though small relative to some previous field campaigns, produced high-fidelity moisture analyses in which sonde precipitable water compared well with independent estimates. An assessment of operational model moisture analyses using corrected sonde data shows an overall good agreement with the exception at upper levels, where model moisture and clouds are more abundant than the sonde data would indicate.

The primary goal of this study is to explore the potential for estimating the vertical velocity (VV) of air from the surface to the stratosphere, using widely available radiosonde and dropsonde data. The rise and fall rates of radiosondes and dropsondes, respectively, are a combination of the VV of the atmosphere and stillair rise-fall rates. The still-air rise-fall rates are calculated using basic fluid dynamics and characteristics of radiosonde and dropsonde systems. This study validates the technique to derive the VV from radiosonde and dropsonde data and demonstrates its value. This technique can be easily implemented by other users for various scientific applications.The technique has been applied to the Terrain-induced Rotor Experiment (T-REX) dropsonde and radiosonde data. Comparisons among radiosonde, dropsonde, aircraft, and profiling radar vertical velocities show that the sonde-estimated VV is able to capture and describe events with strong vertical motions (larger than ;1 m s 21 ) observed during T-REX. The VV below ;5 km above ground, however, is overestimated by the radiosonde data. The analysis of derived VVs shows interesting features of gravity waves, rotors, and turbulence. Periodic variations of vertical velocity in the stratosphere, as indicated by the radiosonde data, correspond to the horizontal wavelength of gravity waves with an averaged horizontal wavelength of ;15 km. Two-dimensional VV structure is described in detail by successive dropsonde deployment.

A GPS dropsonde is a scientific instrument deployed from research and operational aircraft that descends through the atmosphere by a parachute. The dropsonde provides high-quality, high-vertical-resolution profiles of atmospheric pressure, temperature, relative humidity, wind speed, and direction from the aircraft flight level to the surface over oceans and remote areas. Since 1996, GPS dropsondes have been routinely dropped during hurricane reconnaissance and surveillance flights to help predict hurricane track and intensity. From 1996 to 2012, NOAA has dropped 13,681 dropsondes inside hurricane eye walls or in the surrounding environment for 120 tropical cyclones (TCs). All NOAA dropsonde data have been collected, reformatted to one format, and consistently and carefully quality controlled using state-of-the-art quality-control (QC) tools. Three value-added products, the vertical air velocity and the radius and azimuth angle of each dropsonde location, are generated and added to the dataset. As a result, a long-term (1996–2012), high-quality, high-vertical-resolution (∼5–15 m) GPS dropsonde dataset is created and made readily available for public access. The dropsonde data collected during hurricane reconnaissance and surveillance flights have improved TC-track and TC-intensity forecasts significantly. The impact of dropsonde data on hurricane studies is summarized. The scientific applications of this long-term dropsonde dataset are highlighted, including characterizing TC structures, studying TC environmental interactions, identifying surface-based ducts in the hurricane environment that affect electromagnetic wave propagation, and validating satellite temperature and humidity profiling products.

A pilot experiment was conducted in the period from April to June 2008 in the Straits of Florida near Port Everglades, Florida, in order to study the dynamics of far wakes of ships. In this experiment, a small boat with downward-looking sonar made "snakelike" sections through wakes of ships of opportunity during the TerraSAR-X overpasses. The ship and its parameters (length, speed, course, etc.) were identified utilizing an automated identification system. The sonar responded to the clouds of microbubbles generated in the ship wake by the propulsion system and ship-hull turbulence. The ship wakes were traced in the sonar signal typically from 10 to 30 min after the ship's passage. A preliminary analysis of the measurements suggests that the visibility of the centerline ship wake in synthetic aperture radar (SAR) images is correlated with the presence of microbubbles in the wake. This supports the hypothesis that natural surfactants scavenged and brought to the surface by rising bubbles play an important role in the wake visibility in SAR. The influence of the wind-wave field on the ship wake, as well as the effect of screening of the wind-wave field by the ship's hull, adds another level of complexity to wake patterns observed in SAR images.

Abstract. The water vapor distribution in the upper troposphere-lower stratosphere (UTLS) region has a strong impact on the atmospheric radiation budget. Transport and mixing processes on different scales mainly determine the water vapor concentration in the UTLS. Here, we investigate the effect of mountain waves on the vertical transport and mixing of water vapor. For this purpose we analyze measurements of water vapor and meteorological parameters recorded by the DLR Falcon and NSF/NCAR Gulfstream V research aircraft taken during the Deep Propagating Gravity Wave Experiment (DEEPWAVE) in New Zealand. By combining different methods, we develop a new approach to quantify location, direction and irreversibility of the water vapor transport during a strong mountain wave event on 4 July 2014. A large positive vertical water vapor flux is detected above the Southern Alps extending from the troposphere to the stratosphere in the altitude range between 7.7 and 13.0 km. Wavelet analysis for the 8.9 km altitude level shows that the enhanced upward water vapor transport above the mountains is caused by mountain waves with horizontal wavelengths between 22 and 60 km. A downward transport of water vapor with 22 km wavelength is observed in the lee-side of the mountain ridge. While it is a priori not clear whether the observed fluxes are irreversible, low Richardson numbers derived from dropsonde data indicate enhanced turbulence in the tropopause region related to the mountain wave event. Together with the analysis of the water vapor to ozone correlation, we find indications for vertical transport followed by irreversible mixing of water vapor.For our case study, we further estimate greater than 1 W m −2 radiative forcing by the increased water vapor concentrations in the UTLS above the Southern Alps of New Zealand, resulting from mountain waves relative to unperturbed conditions. Hence, mountain waves have a great potential to affect the water vapor distribution in the UTLS. Our regional study may motivate further investigations of the global effects of mountain waves on the UTLS water vapor distributions and its radiative effects.

International audienceConstellations of driftsonde systems— gondolas floating in the stratosphere and able to release dropsondes upon command— have so far been used in three major field experiments from 2006 through 2010. With them, high-quality, high-resolution, in situ atmospheric profiles were made over extended periods in regions that are otherwise very difficult to observe. The measurements have unique value for verifying and evaluating numerical weather prediction models and global data assimilation systems; they can be a valuable resource to validate data from remote sensing instruments, especially on satellites, but also airborne or ground-based remote sensors. These applications for models and remote sensors result in a powerful combination for improving data assimilation systems. Driftsondes also can support process studies in otherwise difficult locations—for example, to study factors that control the development or decay of a tropical disturbance, or to investigate the lower boundary layer over the interior Antarctic continent. The driftsonde system is now a mature and robust observing system that can be combined with flight-level data to conduct multidisciplinary research at heights well above that reached by current research aircraft. In this article we describe the development and capabilities of the driftsonde system, the exemplary science resulting from its use to date, and some future applications

During the THORPEX (The Observing System Research and Predictability Experiment) Pacific Asian Regional Campaign (T‐PARC), from 1 August to 30 September 2008, ∼1900 high‐quality, high vertical resolution soundings were collected over the Pacific Ocean. These include dropsondes deployed from four aircrafts and zero‐pressure balloons in the stratosphere (NCAR's Driftsonde system). The water vapor probability distribution and spatial variability in the northern subtropical Pacific (14°–20°N, 140°E–155°W) are studied using Driftsonde and COSMIC (Constellation Observing System for Meteorology, Ionosphere, and Climate) data and four global reanalysis products. Driftsonde data analysis shows distinct differences of relative humidity (RH) distributions in the free troposphere between the Eastern and Western Pacific (EP and WP, defined as east and west of 180°, respectively), very dry with a single peak of ∼1% RH in the EP and bi‐modal distributions in the WP with one peak near ice saturation and one varying with altitude. The frequent occurrences of extreme dry air are found in the driftsonde data with 59% and 19% of RHs less than or equal to 5% and at 1% at 500 hPa in the EP, respectively. RH with respect to ice in the free troposphere exhibits considerable longitudinal variations, very low (<20%) in the EP, but varying from 20% to 100% in the WP. Inter‐comparisons of Driftsonde, COSMIC and reanalysis data show generally good agreement among the Driftsonde, COSMIC, ECMWF Reanalysis‐Interim (ERA‐Interim) and Japanese Reanalysis (JRA) below 200 hPa. The ERA‐Interim and JRA are approved to be successful on describing RH frequency distributions and spatial variations in the region. The comparisons also reveal problems in Driftsonde, two National Center for Environmental Prediction (NCEP) reanalyses and COSMIC data. The moist layer at 200–100 hPa in the WP shown in the ERA‐Interim, JRA and COSMIC is missing in Driftsonde data. Major problems are found in the RH means and variability over the study region for both NCEP reanalyses. Although the higher‐moisture layer at 200–100 hPa in the WP in the COSMIC data agrees well with the ERA‐Interim and JRA, it is primarily attributed to the first guess of the 1‐Dimensional (1D) variational analysis used in the COSMIC retrieval rather than the refractivity measurements. The limited soundings (total 268) of Driftsonde data are capable of portraying RH probability distributions and longitudinal variability. This implies that Driftsonde system has the potential to become a valuable operational system for upper air observations over the ocean.