the magnitude of the gain. Thus, the delay time of -0.5 s observed in Fig. 4 increases to 12 s at E, = 46 V/pin. Furthermore, the use of additional polymer layers will not decrease the threshold for oscillation noticeably unless an index-matching liquid is used to reduce the reflection losses accordingly. Once oscillation has been achieved, however, the P C reflectivity will increase significantly faster with EL, for a larger multilayer stack.Finally, predicting the threshold for oscillation or the value of R from measurements of tw o-beam coupling galn is not a simple matter. Recent work has shown that these ~o l v -L , mers benefit from gain enhanceinent due to very slow motion of the index eratine, which -l n e k that the cavity beam is expected to be frequency-shifted from the pumping beam. This complicates the theoretical analysis to a level beyond the scope of this report (25).
Antibodies generated by candidate HIV-1 vaccines in a phase I clinical trial were assessed for neutralizing activity with a panel of eight well-characterized, genetically diverse clade B primary isolates having an R5 phenotype. The vaccines consisted of one of three different recombinant canarypox vectors expressing membrane-anchored HIV-1(MN)gp120 (ALVAC vCP205, vCP1433, and vCP1452) followed by boosting with a soluble gp160 hybrid consisting of MNgp120 and the majority of gp41 from strain IIIB. Serum samples from a subset of volunteers in each arm of the trial, containing moderate to high titers of neutralizing antibodies to HIV-1 MN, were analyzed. Competition assays with peptides revealed that the majority of neutralizing activity was specific for the MN-V3 loop. Despite MN-specific neutralization titers that sometimes exceeded 1:500, no neutralization of primary isolates was detected and, in some cases, mild infection enhancement was observed. In addition, little or no neutralization of the HIV-1 IIIB heterologous T cell line-adapted strain of virus was detected. These results reinforce the notion that monovalent HIV-1 ENV is a poor immunogen for generating cross-reactive neutralizing antibodies.
The hydration of polar natural and synthetic lipids
yields a variety of lipid phases including various
inverted
cubic phases and the inverted hexagonal (HII) phase.
The HII phase can be considered as aqueous columns
encased
with a monolayer of lipids and arranged in a hexagonal pattern.
The polar head groups are well-ordered at the water
interface, whereas the lipid tails are disordered to fill the volume
between the tubes of water. A particularly
interesting
characteristic of the HII phase is the large temperature
effect on the basis vector length d of the hexagonal
lattice.
Previous studies indicate that polymerization of the lipid region
of the HII phase might reduce the sensitivity of
the
basis vector to temperature. A phosphoethanolamine (PE) was
designed and synthesized with dienoyl groups in
each lipid tail in an attempt to cross-link the lipids around and along
the water core of the HII phase. The
synthesis
of the the PE was accomplished by acylation of
3-(4-methoxybenzyl)-sn-glycerol with
2,4,13-(E,E,Z)-docosatrienoic
acid, followed by deprotection, then phosphorylation with
dichloro-[[N-[(2,2,2-trichloroethoxy)carbonyl]-2-amino]ethyl]phosphinic acid to give the Troc-PE, which was converted to
the PE with activated zinc and acetic acid. The
hydrated PE (1/1 weight lipid/water) formed the HII phase
over an extended temperature range. Polymerization to
high conversion was accomplished at 60 °C with the aid of redox
initiators. Polymerization was followed in-situ
using X-ray diffraction over a period of 48 h. The scattering,
which weakened over the course of the reaction,
remained consistent with a hexagonal phase. Temperature cycling of
the polymerized HII phase showed an unaltered
pattern on decreasing temperature while maintaining the same lattice
parameter, unlike that of the unpolymerized
phase where the value increased with decreasing temperature. Thus
it is possible to fix the dimensions of the
HII
phase by cross-linking polymerization of appropriately designed
reactive lipids.
Several attempted syntheses of Ti-TMS1, a hexagonal
mesoporous TiO2 reported by Antonelli and Ying, have
resulted in a lamellar structure as determined by two-dimensional
powder X-ray diffraction and transmission electron microscopy (TEM).
Regions of partially calcined lamellar materials, when observed by
TEM can be mistaken for hexagonal material. In no cases are
specimens produced that were unambiguously hexagonal. It is
concluded that the hexagonal material exists, if at all, only as a
minor component of a larger lamellar structure when phosphate
surfactants are used. Ti-TMS1 therefore remains
elusive.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.