The penultimate step of prokaryotic coenzyme A (CoA) biosynthesis is directed by the essential enzyme phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3), an attractive target for antibiotics. The reaction catalyzed by PPAT is rate-limiting and involves the transfer of an adenylyl group from ATP to 4 H -phosphopantetheine to form 3 H -dephospho-CoA. Rhombohedral crystals of PPAT from Mycobacterium tuberculosis (Rv2965c) were obtained. The crystals belong to space group R32, with unit-cell parameters a = 68.69 A Ê , = 91.81 . The crystals diffract to better than 2 A Ê resolution on a Cu K rotating-anode generator. The packing density for one polypeptide chain in the asymmetric unit is 2.89 A Ê 3 Da À1 , with a solvent content of 0.57.
Our exploratory study provides several dissemination approaches that should be considered by practitioners as they seek to promote physical activity in the populations they serve.
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