The Antarctic Circumpolar Current is an important component of the global climate system connecting the major ocean basins as it flows eastward around Antarctica, yet due to the paucity of data, it remains unclear how much water is transported by the current. Between 2007 and 2011 flow through Drake Passage was continuously monitored with a line of moored instrumentation with unprecedented horizontal and temporal resolution. Annual mean near‐bottom currents are remarkably stable from year to year. The mean depth‐independent or barotropic transport, determined from the near‐bottom current meter records, was 45.6 sverdrup (Sv) with an uncertainty of 8.9 Sv. Summing the mean barotropic transport with the mean baroclinic transport relative to zero at the seafloor of 127.7 Sv gives a total transport through Drake Passage of 173.3 Sv. This new measurement is 30% larger than the canonical value often used as the benchmark for global circulation and climate models.

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In situ measurements are used, together with sea surface height data, to study the development and variability of the Great Whirl (GW), a large quasi‐stationary anticyclone that appears off the coast of Somalia during the southwest monsoon season. We find that anticyclonic circulation indicative of the GW appears on average in April, almost two months before the onset of the southwest monsoon winds. This early initiation is coincident with the arrival of annual Rossby waves at the western boundary. With the onset of the monsoon winds in early June, the GW‐proper intensifies quickly, remaining at its peak throughout July, August, and September, and dissipating about one month after the winds have died. The GW is present for an average 166 ± 30 days per year and the position of its northern flank, close to 9°N, coincides with the latitude of zero wind stress curl. The intraseasonal variability of the GW is intense as a result of mutual advection with one to three flanking cyclones, which accompany the GW 70% of the time and tend to circulate clockwise around it. There is no consistent seasonal pattern for the development or dissipation of the GW, although movement to the southwest is common toward the end of the season. The GW of 1995 deepened from 200 m in June to over 1000 m in September, and strengthened from a swirl transport of 10 to 60 Sv. Cool waters in its core resulted from advection via the Somali Current and some local vertical mixing.

Signal transducer and activator of transcription (STAT) proteins are involved in hematopoietic cytokine receptor signaling pathways that regulate cell proliferation, differentiation, and survival. STATs are dysregulated in acute myeloid leukemia (AML); mechanisms of dysregulation include constitutive activation and truncation of the C-terminal transactivation domain; the latter results in a ␤ isoform that has a trans-dominant negative effect on gene induction mediated by the fulllength STAT␣ form. It was hypothesized that constitutive STAT activity might correlate with unfavorable treatment outcome in AML. Pretreatment bone marrow samples from 63 adult patients with AML were analyzed by electrophoretic mobility shift assay for the presence of STAT DNA-binding activity. Isoforms and relative levels of STAT proteins were determined by immunoblotting. Constitutive STAT3 activity was detected in samples from 28 (44%) patients. Pretreatment clinical characteristics, expression of STAT␣/␤ isoforms, and treatment regimens did not differ significantly between patients with and without constitutive STAT3 activity. Disease-free survival (DFS) was significantly shorter in patients with than in patients without constitutive STAT3 activity (median 8.7 vs 20.6 months; P ‫؍‬ .01).Overall survival did not differ significantly. The subgroup of patients with constitutive STAT3 activity and the STAT3␤ isoform had the shortest DFS (P ‫؍‬ .006) and shorter overall survival (P ‫؍‬ .049) than all other patients. Whether adverse treatment outcome is attributable to constitutive STAT activity itself or to a process that leads to constitutive STAT activity remains to be determined. This is the first demonstration of a prognostic significance for STAT proteins in a malignancy. (Blood. 2002;99:252-257)

International audienceDrake Passage is the narrowest constriction of the Antarctic Circumpolar Current (ACC) in the Southern Ocean, with implications for global ocean circulation and climate. We review the long-term sustained monitoring programs that have been conducted at Drake Passage, dating back to the early part of the twentieth century. Attention is drawn to numerous breakthroughs that have been made from these programs, including (1) the first determinations of the complex ACC structure and early quantifications of its transport; (2) realization that the ACC transport is remarkably steady over interannual and longer periods, and a growing understanding of the processes responsible for this; (3) recognition of the role of coupled climate modes in dictating the horizontal transport and the role of anthropogenic processes in this; and (4) understanding of mechanisms driving changes in both the upper and lower limbs of the Southern Ocean overturning circulation and their impacts. It is argued that monitoring of this passage remains a high priority for oceanographic and climate research but that strategic improvements could be made concerning how this is conducted. In particular, long-term programs should concentrate on delivering quantifications of key variables of direct relevance to large-scale environmental issues: In this context, the time-varying overturning circulation is, if anything, even more compelling a target than the ACC flow. Further, there is a need for better international resource sharing and improved spatiotemporal coordination of the measurements. If achieved, the improvements in understanding of important climatic issues deriving from Drake Passage monitoring can be sustained into the future

The Kuroshio Extension System Study (KESS) aimed to quantify processes governing the variability of and the interaction between the Kuroshio Extension and the recirculation gyre. To meet this goal, a suite of instrumentation, including 43 inverted echo sounders equipped with bottom pressure gauges and current meters [current and pressure recording inverted echo sounders (CPIES)], was deployed. The array was centered on the first quasi-stationary meander crest and trough east of Japan, which is also the region of highest eddy kinetic energy. KESS was the first experiment to deploy a large quantity of these new CPIES instruments, and it was unique in that the instruments were deployed in water depths (5300-6400 m) close to their limit of operation. A comprehensive narrative of the methodology to produce mesoscale-resolving fourdimensional circulation fields of temperature, specific volume anomaly, and velocity from the KESS CPIES array is provided. In addition, an improved technique for removing pressure drift is introduced. Methodology and error estimates were verified with several independent datasets. Temperature error was lowest on the equatorward side of the Kuroshio Extension core and decreased with depth (1.58C at 300 m, 0.38C at 600 m, and ,0.18C below 1200 m). Velocity errors were highest in regions of strong eddy kinetic energy, within and south of the jet core. Near the surface, the error in geostrophic velocity between adjacent CPIES was typically 10 cm s 21 , decreasing downward to 6 cm s 21 at 500-m depth and 5 cm s 21 below 800 m. The rms differences from pointwise current measurements are nearly twice as large as the geostrophic errors, because the pointwise velocities include submesoscale and ageostrophic contributions.

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The use of flow cytometry to diagnose hematological malignancies has become routine due to its ability to often differentiate between morphologically similar diseases based on antigens expressed on the surface of malignant cells. In an attempt to expand on the utility of flow cytometry in the study of B-cell malignancies we have used the most reliable quantitative methodology, QIFI (quantitative indirect immunofluorescence assay), to study the expression of CD5, CD10, CD11c, CD19, CD20, CD22, CD23, and CD79b in 384 cases of several common B-lineage malignancies, including: B-ALL, CLL, SLL, hairy cell leukemia, diffuse large B-cell lymphoma, and follicular lymphoma. The impetus behind this extensive, single institution study of surface antigens was two-fold: evaluating similarities and differences of antigen expression between B-cell neoplasms and finding additional clinical utility for the quantitative flow cytometric data generated. Our results show that each distinct malignant histology has its own quantitative pattern of surface antigen expression. In most cases, these quantitative patterns do not increase the ability of flow cytometry to distinguish between them. However, a high expression of specific antigens on a given B-cell malignancy may potentially identify optimal therapeutic targets for current and/or future monoclonal antibody-based therapies.