Otolith chemistry is frequently employed in the reconstruction of fish environmental histories. While some elements have been strongly correlated with environmental factors (e.g. salinity, temperature, water chemistry), others may not indicate exogenous factors and simply add endogenous variability to a data set. Several commonly assessed elements were previously identified as being only present in the proteinaceous fraction of endolymph from black bream Acanthopagrus butcheri, suggesting that the choice of elements in otolith multi-elemental fingerprinting could influence their utility as natural environmental markers. To test this hypothesis, we performed several cluster analyses based on different sets of trace element data extracted from both the core and larval region of otoliths of juvenile black bream. We clustered in 3 different ways: (1) all elements analysed; (2) elements identified as being primarily in the salt fraction of the endolymph (i.e. inorganic); and (3) elements identified as being associated with the protein fraction of the endolymph. We subsequently assessed the power of the resulting clusters to resolve cohort identity and/or collection location based on differences in otolith chemistry using multinomial logistic regression. Our results indicate that clustering based solely on salt-fraction elements is best for resolving spatio-temporal variability in spawning sources and larval nursery habitats of black bream.
Exploratory GIS models present multiple different conceptual versions of space. This article focusses on the landscape level pathways between areas defined as suitable for land use and occupancy within the Willandra Lakes Region World Heritage Area (WLRWHA), New South Wales (NSW), Australia. Models of the potential connections between ecologically significant land use patches and key hydrology provide iterative networks of functional connectivity, highlighting salient pathways of past land use and occupancy of Country. The shape of the connections between places is important to understanding Country from the inside. Outputs from these network models are a powerful visualisation tool because they display areas where contact with the 19 th c Europeans, particularly through fence construction and ground water appropriation, caused greater levels of exploitation and damage than currently recognised. Concomitantly, the benefit of situating these network techniques within an exploratory framework cannot be understated. The iterative nature of the exploratory design allows for multiple presentations of the connectivity between the spaces within the WLRWHA and therefore multiple ways of knowing and seeing space. Modelling the potential pathways between suitable patches opens the door to discussions about the diverse possible corridors of activity within pre-European settlement of Country and the corollary discussion of how European settlement substantially impacted upon these connections and continues to impact on a living Country. Paakantji (Barkindji), Mutthi Mutthi, and Ngyiampaa communities for sharing oral testimonies, enabling research on their homelands, and supporting our work. Further acknowledgements are to NSW Parks staff and individual community members (past and present
Defining spaces and places in retrojective Geographical Information Systems (GIS) of land use and occupancy is not an easy task. Research into the areas described as suitable for land use and occupancy need to incorporate multiple perspectives of what makes a land use patch useful or salient ecologically. The effect of the concept of 'Terra Nullius' and European colonisation is deeply apparent in the current GIS models of historical land use and occupancy of Aboriginal communities within arid zones in Australia. Biocultural zones of land use and occupancy zones omit spaces and places of habitation due to European bias of what a suitable ecological or hydrological land use zone should look like. This article employs Exploratory GIS methods to interrogate the data layers within the Willandra Lakes Region World Heritage Area, NSW, Australia. This work conclusively demonstrates that there are ranges of areas and land suitability zones prior to colonisation in the nineteenth century. In turn, these Exploratory GIS models of an active Country comprehensively address the question of why visually salient areas of hydrological and ecological Indigenous land use and occupancy continue to be ignored, destroyed, and damaged by settlements in semi-arid regions. Biocultural GIS mapping unpacks the myth that areas were empty or uninhabited by Aboriginal communities and underlines the need for biocultural GIS mapping tools to understand the habitable spaces and places of the arid zone.
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