Stimulating cells by using light is a non-invasive technique that provides flexibility in probing different locations while minimizing unintended effects on the system. We propose a new way to make cells photosensitive without using genetic or chemical manipulation, which alters natural cells, in conjunction with Quantum Dots (QDs). Remote switching of cellular activity by optical QD excitation is demonstrated by integrating QDs with cells: CdTe QD films with prostate cancer (LnCap) cells, and CdSe QD films and probes with cortical neurons. Changes in membrane potential and ionic currents are recorded by using the patch-clamp method. Upon excitation, the ion channels in the cell membrane were activated, resulting in hyperpolarization or depolarization of the cell.
We report integration of CdTe quantum dot (QD) film with LnCap (prostate cancer) cell and CdSe QD probes with cortical neurons for control of cellular activity. We demonstrate the remote switching of cellular activity by exciting QDs with light. Changes in membrane potential and ionic currents are recorded using the patch-clamp method. Upon excitation, the cell shows activation of ion channels and hyperpolarization of the cell membrane.
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