Dietary supplementation with beetroot juice (BR) has been shown to reduce resting blood pressure and the O(2) cost of submaximal exercise and to increase tolerance to high-intensity cycling. We tested the hypothesis that the physiological effects of BR were consequent to its high NO(3)(-) content per se, and not the presence of other potentially bioactive compounds. We investigated changes in blood pressure, mitochondrial oxidative capacity (Q(max)), and physiological responses to walking and moderate- and severe-intensity running following dietary supplementation with BR and NO(3)(-)-depleted BR [placebo (PL)]. After control (nonsupplemented) tests, nine healthy, physically active male subjects were assigned in a randomized, double-blind, crossover design to receive BR (0.5 l/day, containing ∼6.2 mmol of NO(3)(-)) and PL (0.5 l/day, containing ∼0.003 mmol of NO(3)(-)) for 6 days. Subjects completed treadmill exercise tests on days 4 and 5 and knee-extension exercise tests for estimation of Q(max) (using (31)P-magnetic resonance spectroscopy) on day 6 of the supplementation periods. Relative to PL, BR elevated plasma NO(2)(-) concentration (183 ± 119 vs. 373 ± 211 nM, P < 0.05) and reduced systolic blood pressure (129 ± 9 vs. 124 ± 10 mmHg, P < 0.01). Q(max) was not different between PL and BR (0.93 ± 0.05 and 1.05 ± 0.22 mM/s, respectively). The O(2) cost of walking (0.87 ± 0.12 and 0.70 ± 0.10 l/min in PL and BR, respectively, P < 0.01), moderate-intensity running (2.26 ± 0.27 and 2.10 ± 0.28 l/min in PL and BR, respectively, P < 0.01), and severe-intensity running (end-exercise O(2) uptake = 3.77 ± 0.57 and 3.50 ± 0.62 l/min in PL and BL, respectively, P < 0.01) was reduced by BR, and time to exhaustion during severe-intensity running was increased by 15% (7.6 ± 1.5 and 8.7 ± 1.8 min in PL and BR, respectively, P < 0.01). In contrast, relative to control, PL supplementation did not alter plasma NO(2)(-) concentration, blood pressure, or the physiological responses to exercise. These results indicate that the positive effects of 6 days of BR supplementation on the physiological responses to exercise can be ascribed to the high NO(3)(-) content per se.

These results suggest that acute dietary nitrate supplementation with 0.5 L of BR improves cycling economy, as demonstrated by a higher PO for the same VO2 and enhances both 4- and 16.1-km cycling TT performance.

Exercise intensity has traditionally been described, prescribed and normalised as a fraction (%) of the maximal oxygen uptake ( V˙O (2max)). We hypothesised that the extent of inter-subject variability in the physiological responses to exercise would be greater when work rates were prescribed using % V˙O (2max) as compared to % 'delta' (Δ), a method of normalising exercise intensity in which both the gas exchange threshold (GET) and the V˙O (2max) are considered. 9 men completed a ramp incremental test on a cycle ergometer to establish the GET and V˙O (2max). Subsequently, subjects completed 6 constant-work-rate exercise bouts at intensities corresponding to: 50%, 70% and 90% V˙O (2max); and 60% GET, 40% Δ (that is, 40% of the difference between the GET and V˙O (2max)) and 80% Δ. For all bouts, exercise was continued for 20 min or until task failure if this occurred sooner. When exercise was prescribed using the % Δ concept, there were significant reductions in the inter-subject variability in pulmonary gas exchange, blood lactate accumulation, heart rate, and ratings of perceived exertion (all P < 0.05). In conclusion, the % Δ concept resulted in more consistent inter-subject physiological responses to constant-work-rate exercise and should be used in preference to % V˙O (2max) to more effectively normalise exercise intensity.

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