Migration inhibitory factor (MIF) was produced by lymphoid cells of Lewis rats in response to infection with Bacille Calmette Guèrin (BCG), mixed lymphocyte reaction or concanavalin A stimulation. It was found that rat lymphokine-containing supernatants demonstrate a definite species specificity, in that MIF activity is observed only when rat indicator cells and not when guinea-pig indicator cells are used. A comparative study of MIF activities, using rat peritoneal exudate cells, revealed that all three methods stimulated MIF production by either spleen or lymph node cells. Only infection with BCG induced peripheral blood leukocytes to elaborate MIF. In all cases, rat MIF inhibited rat peritoneal exudate cells but not guinea-pig peritoneal exudate cells. Possible explanations for the species specificity of rat MIF including a role for receptor carbohydrate moieties and fibronectin are discussed.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.