RNA–protein interactions are key to many aspects
of cellular
homeostasis and their identification is important to understanding
cellular function. Multiple strategies have been developed for the
RNA-centric characterization of RNA–protein complexes. However,
these studies have all been done in immortalized cell lines that do
not capture the complexity of heterogeneous tissue samples. Here,
we develop hybridization purification of RNA–protein complexes
followed by mass spectrometry (HyPR-MS) for use in tissue samples.
We isolated both polyadenylated RNA and the specific long noncoding
RNA MALAT1 and characterized their protein interactomes. These results
demonstrate the feasibility of HyPR-MS in tissue for the multiplexed
characterization of specific RNA–protein complexes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.