The
zebrafish embryo (Danio rerio) has
developed into one of the most important nonsentient animal
models for the hazard assessments of chemicals, but the processes
governing its toxicokinetics (TK) are poorly understood. This study
compares the uptake of seven test compounds into the embryonic body
and the yolk sac of the zebrafish embryo using TK experiments, a dialysis
approach, thermodynamic calculations, and kinetic modeling. Experimental
data show that between 95% (4-iodophenol) and 67% (carbamazepine)
of the total internal amount in 26 h post fertilization (hpf) embryos
and between 80 and 49% in 74 hpf embryos were found in the yolk. Thus,
internal concentrations determined for the whole embryo overestimate
the internal concentration in the embryonic body: for the compounds
of this study, up to a factor of 5. Partition coefficients for the
embryonic body and a one-compartment model with diffusive exchange
were calculated for the neutral test compounds and agreed reasonably
with the experimental data. For prevalently ionic test compounds at
exposure pH (bromoxynil, paroxetine), however, the extent and the
speed of uptake were low and could not be modeled adequately. A better
understanding of the TK of ionizable test compounds is essential to
allow assessment of the validity of this organismic test system for
ionic test compounds.
Svalbard is an important study area for investigating the long-range transport of mercury (Hg) and other trace elements to the Arctic. Few studies have focused on their concentrations in Arctic soils. With ongoing climate change leading to thawing permafrost ground the soil compartment is of increasing importance in the Arctic. In this study, elemental composition and soil organic matter (SOM) content of surface and mineral soils in Svalbard are presented. The aim is to provide new data on soils in the Arctic and to gain more knowledge about the role of the soil in the biogeochemical cycle of mercury (Hg). Concentrations are reported for Al, As, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, S and Zn. Samples were taken in Adventdalen and in the area near Ny-Ålesund. We obtained a mean Hg concentration of 0.111 ± 0.036 μg/g in surface soils (range 0.041-0.254 μg/g). Hg levels in mineral soils (mean: 0.025 ± 0.013 μg/g; range: 0.004-0.060 μg/g) were substantially lower than in the corresponding surface soils. Hg strongly accumulates in the surface soil layer (upper 3 cm) and is associated with SOM (surface soil: 59 ± 14%). Hg concentrations in the surface soil were slightly lower than those in the humus layer in mainland Norway and were comparable to levels in soils elsewhere in the Arctic. An inverse association of Hg was found with elements attributed to the mineral soil, indicating that Hg is predominantly derived from atmospheric deposition.
The zebrafish embryo is an important model in ecotoxicology but the spatial distribution of chemicals and the relation to observed effects is not well understood. Quantitative imaging can help to gain insights into the distribution of chemicals in the zebrafish embryo. Laser ablation inductively coupled plasma-mass spectrometry (LA-ICP-MS) is used to quantify the uptake and the uptake kinetics of the bromine (Br) containing organophosphate naled (Dibrom®, dimethyl-1,2-dibromo-2,2-dichloroethylphosphate) and its distribution in zebrafish embryos using Br as the marker element. During exposure, the Br amounts increase in the embryos parallel to the irreversible inhibition of the acetylcholinesterase (AChE). The final amount of Br in the embryo (545 pmol/embryo) corresponds to a 280-fold enrichment of naled from the exposure solution. However, LC-MS/MS analyses showed that the internal concentration of naled remained below the LOD (7.8 fmol/embryo); also the concentration of its known transformation product dichlorvos remained low (0.85 to 2.8 pmol/embryo). These findings indicate the high reactivity and high transformation rate of naled to other products than dichlorvos. 12C normalized intensity distributions of Br in the zebrafish embryo showed an enrichment of Br in its head region. Kernel density estimates of the LA-ICP-MS data were calculated and outline the high reproducibility between replicated and the shift in the Br distribution during exposure. The Br enrichment indicates a preferential debromination or direct covalent reaction of naled with AChE in this region.
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Electronic supplementary materialThe online version of this article (10.1007/s00216-018-1471-2) contains supplementary material, which is available to authorized users.
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