(1) Background: This study aimed to detect feline coronavirus (FCoV) and characterize spike (S) gene mutation profiles in cats suffering from diseases other than feline infectious peritonitis (FIP) using commercial real-time reverse transcription polymerase chain reaction (RT-qPCR) and reevaluating results by sequencing. (2) Methods: In 87 cats in which FIP was excluded by histopathology and immunohistochemistry, FCoV 7b gene and S gene mutation RT-qPCR was performed prospectively on incisional biopsies and fine-needle aspirates of different organs, body fluids, and feces. Samples positive for S gene mutations or mixed FCoV underwent sequencing. (3) Results: In 21/87 cats, FCoV RNA was detectable. S gene mutations were detected by commercial RT-qPCR (and a diagnostic algorithm that was used at the time of sample submission) in at least one sample in 14/21 cats (66.7%), with only mutated FCoV in 2/21, only mixed in 1/21, and different results in 11/21 cats; in the remaining 7/21 cats, RNA load was too low to differentiate. However, sequencing of 8 tissue samples and 8 fecal samples of 9 cats did not confirm mutated FCoV in any of the FCoV RNA-positive cats without FIP. (4) Conclusions: Sequencing results did not confirm results of the commercial S gene mutation RT-qPCR.
Anti-Müllerian hormone (AMH), known for its role during foetal sexual differentiation, is secreted by the Sertoli cells in males and the granulosa cells in females during post-natal life. As serum AMH concentrations correlate with follicle numbers, AMH is utilized as a marker of ovarian reserve in many species. In dogs and cats, AMH is used as a diagnostic tool to determine spay or neuter status. In the available literature, no research regarding serum AMH levels in rabbits has been published yet. The objectives of the present study were to (1) measure serum AMH concentrations in female rabbits and investigate the value of AMH as a diagnostic tool to differentiate between
Im Laufe der vergangenen beiden Jahre hat eine wachsende Zahl der wissenschaftlichen Bibliotheken in Deutschland damil begonnen, die Idee der "digitalen Bibliothek" zu verwirklichen. Der Aufsatz gibt einen Überblick über die derzeit in der Universitätsbibliothek Augsburg laufenden Digitalisierungs-Projekte. Primäre Intention der Projekte ist die Erweiterung des Dienstleistungsspektrums der Bibliothek und die Verbesserung der Informations-Infrastruktur im wissenschaftlichen Bereich durch den direkten Transfer von digitalen Informationen und Dokumenten an den PC-Arbeitsplatz des Wissenschaftlers. Die den Projekten zugrundeliegenden Konzepte unterscheiden sich hinsichtlich ihrer Inhalte und Zielsetzungen. Der Aufsatz beschäftigt sich speziell mit Fragen der retrospektiven Digitalisierung und zeigt in den Projekten gesammelte praktische Erfahrungen auf.
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