Aim A conspicuous climatic and biogeographical transition occurs at 40-45°N in western North America. This pivot point marks a north-south opposition of wet and dry conditions at interannual and decadal time-scales, as well as the northern and southern limits of many dominant western plant species.Palaeoecologists have yet to focus on past climatic and biotic shifts along this transition, in part because it requires comparisons across dissimilar records [i.e. pollen from lacustrine sediments to the north and plant macrofossils from woodrat (Neotoma) middens to the south]. To overcome these limitations, we are extending the woodrat-midden record northward into the lowlands of the central Rocky Mountains.Location Woodrat middens were collected from crevices and rock shelters on south-facing slopes of Dutch John Mountain (2000-2200 m, 40°57¢ N, 109°25¢ W), situated on the eastern flanks of the Uinta Mountains in northeastern Utah. The site is near the regional limits for Pinus ponderosa, P. edulis, P. contorta, Cercocarpus ledifolius var. intricatus, Abies concolor, Ephedra viridis and other important western species.Methods We analysed pollen and plant macrofossils from the 40,000-year midden sequence. The middens represent brief, depositional episodes (mostly years to decades). Four middens represent the early to full-glacial period (40,000-18,000 cal-yr bp), eight middens are from the late-glacial/early Holocene transition (13,500-9000 cal yr bp), and 33 middens span the mid-to-late Holocene (last 7500 years). Temporal density of our Holocene middens (one every c. 210 years) is comparable with typical Holocene pollen sequences from lake sediments.Results Early to full-glacial assemblages are characterized by low diversity and occurrence of montane conifers (Picea pungens, Pseudotsuga menziesii, P. flexilis, Juniperus communis) absent from the site today. Diversity increases in the lateglacial samples with the addition of J. scopulorum, J. horizontalis, C. montanus, C. ledifolius var. intricatus and mesic understory species. The coniferous trees and J. communis declined and J. osteosperma appeared during the late-glacial/ Holocene transition. Juniperus osteosperma populations have occupied the site throughout the Holocene. Pinus ponderosa was established by 7500 cal-yr bp, and has occurred at least locally ever since. Montane conifers and J. horizontalis persisted until c. 5500 cal-yr bp. The signature events of the late Holocene were the invasions of P. edulis and Ephedra viridis and establishment of pinyon-juniper woodland in the last 800 years.Main conclusions The Dutch John Mountain midden record adds to an emerging picture in which mid-elevation conifers (P. flexilis, Pseudotsuga menziesii, Picea pungens, J. scopulorum, J. communis) dominated vegetation over