The success of MMS depends on high-quality tissue sections. The staining process should be optimized to be reproducible and reliable. To readily identify and resolve poor staining quality, a firm understanding of the principles upon which tissue staining is based and its pitfalls is necessary.
Flash freezing in a histobath produced a more rapidly opacified (frozen) specimen ready for cryotomy, expediting slide TAT. Tissue histology also demonstrated better quality and minimized freeze artifact.
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