Metabolic reprogramming in skeletal muscles in the human and animal models of amyotrophic lateral sclerosis (ALS) may be an important factor in the diseases progression. We hypothesized that swim training, a modulator of cellular metabolism via changes in muscle bioenergetics and oxidative stress, ameliorates the reduction in muscle strength in ALS mice. In this study, we used transgenic male mice with the G93A human SOD1 mutation B6SJL-Tg (SOD1G93A) 1Gur/J and wild type B6SJL (WT) mice. Mice were subjected to a grip strength test and isolated skeletal muscle mitochondria were used to perform high-resolution respirometry. Moreover, the activities of enzymes involved in the oxidative energy metabolism and total sulfhydryl groups (as an oxidative stress marker) were evaluated in skeletal muscle. ALS reduces muscle strength (−70% between 11 and 15 weeks, p < 0.05), modulates muscle metabolism through lowering citrate synthase (CS) (−30% vs. WT, p = 0.0007) and increasing cytochrome c oxidase and malate dehydrogenase activities, and elevates oxidative stress markers in skeletal muscle. Swim training slows the reduction in muscle strength (−5% between 11 and 15 weeks) and increases CS activity (+26% vs. ALS I, p = 0.0048). Our findings indicate that swim training is a modulator of skeletal muscle energy metabolism with concomitant improvement of skeletal muscle function in ALS mice.

We tested the hypothesis that swim training reverses the impairment of Akt/FOXO3a signaling, ameliorating muscle atrophy in ALS mice. Transgenic male mice B6SJL-Tg (SOD1G93A) 1Gur/J were used as the ALS model (n = 35), with wild-type B6SJL (WT) mice as controls (n = 7). ALS mice were analyzed before ALS onset, at ALS onset, and at terminal ALS. Levels of insulin/Akt signaling pathway proteins were determined, and the body and tibialis anterior muscle mass and plasma creatine kinase. Significantly increased levels of FOXO3a in ALS groups (from about 13 to 21-fold) compared to WT mice were observed. MuRF1 levels in the ONSET untrained group (12.0 ± 1.7 AU) were significantly higher than in WT mice (1.12 ± 0.2 AU) and in the BEFORE ALS group (3.7 ± 0.9 AU). This was associated with body mass and skeletal muscle mass reduction. Swim training significantly ameliorated the reduction of skeletal muscle mass in both TERMINAL groups (p < 0.001) and partially reversed changes in the levels of Akt signaling pathway proteins. These findings shed light on the swimming-induced attenuation of skeletal muscle atrophy in ALS with possible practical implications for anti-cachexia approaches.

BackgroundThe aim of the study was to analyse sports results and the somatic composition of participants in the 2014 FIVB Volleyball Men's World Championship held in Poland. Material/MethodsResearch material involved volleyball players participating in the World Championships (n = 280). Their age, height, weight, one-arm spike reach, both-arm block reach, and Rohrer's index were analysed. The mean value of the features and their derivatives in individual teams and groups were calculated and verified using statistical tests (t-Student, Mann Whitney and Cochran-Cox) if the differences were significant. ResultsThe subjects' mean age was 26.33 years (SD = 3.90), body height 198.41 cm (SD = 6.05), body weight 89.40 kg (SD = 8.43), spike reach 344.08 cm (SD = 13.37), block reach 326.68 cm (SD = 13.09), Rohrer's index 1.15 (SD = 0.10). 90.36% of the volleyball players manifested the leptosomatic body composition, 9.28% the athletic one, and 0.36% the pyknic one. The highest and the lowest ranked teams in the analysed tournament significantly differed in the spike reach (P < 0.005) and in body height (P < 0.015). ConclusionsThe leptosomatic body composition type is dominant among volleyball players at the top level of sports championship. The body height and the spike reach significantly differentiate the top and the bottom ranked teams participating in the 2014 FIVB Volleyball Men's World Championship. These characteristics should be taken into account in selection to play men's volleyball.

Open Access License:This is an open access article distributed under the terms of the Creative Commons Attribution-Non-commercial 4.0 International (http://creativecommons.org/licenses/by-nc/4.0/), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non-commercial and is otherwise in compliance with the license. abstract BackgroundTheaimofthestudywastodeterminedifferencesintheefficiencyofexecutingtechnical actionsbyfemalevolleyballplayerstakingintoaccounttheirpositionsonthecourt. Material/MethodsTheresearchmaterialcomprisedresultsoftheefficiencyoffemalevolleyballplayers'technical actions(n=98)participatinginthe2015Women'sEuropeanVolleyballChampionship.The subjects were divided into five groups: receivers, middle blockers, spikers, setters, and liberoes.Thefollowingtechnicalactionswereanalysed:attack,block,serve,andreceiving aserve.Inordertodeterminethedifferencesbetweenthestudiedgroups,statisticaltests wereused.TheScheffetestwasusedtocomparetheefficiencyinattack,block,andserve. Student'sT-testwasusedtocomparereceivingtheserve. ResultsMiddleblockersaremoreefficientinattackthanspikersby6.82%(p<0.05)andreceivers by 9.61% (p < 0.001). Middle blockers also gain more points in the block per set than receivers(Δx=0.39,p<0.001),setters(Δx=0.49,p<0.001)andspikers(Δx=0.35, p<0.01).Liberoespresenttheefficiencyinreceivingtheservehigherby6.07%(p<0.05) thanreceivers. ConclusionsEfficiencyinattack,blockandreceivingaservesignificantlydiversifiesplayersatdifferent positions. Their positions on the court do not affect the efficiency of the serve. These differencesmayarisefromthegametacticsoftheteamsandmayberelatedtodifferent tasksrealisedatparticularpositions.

In this study, we aim to verify whether swim training can improve lactate metabolism, NAD+ and NADH levels, as well as modify the activity of glycolytic and NADH shuttle enzymes and monocarboxylate transporters (MCTs) in skeletal muscle of amyotrophic lateral sclerosis (ALS) mice. ALS mice (SOD1G93A) (n = 7 per group) were analyzed before the onset of ALS, at first disease symptoms (trained and untrained), and the last stage of disease (trained and untrained), and then compared with a wild-type (WT) group of mice. The blood lactate and the skeletal muscle concentration of lactate, NAD+ and NADH, MCT1 and MCT4 protein levels, as well as lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) activities in skeletal muscle were determined by fluorometric, Western blotting, liquid chromatography-MS3 spectrometry, and spectrometric methods. In the untrained terminal ALS group, there were decreased blood lactate levels (p < 0.001) and increased skeletal muscle lactate levels (p < 0.05) as compared with a WT group of mice. The amount of nicotinamide adenine dinucleotides in the ALS groups were also significantly reduced as well as LDH activity and the level of MCT1. Swim training increased lactate levels in the blood (p < 0.05 vs. ALS TERMINAL untrained). In addition, cytosolic MDH activity and the cMDH/LDH 2.1 ratio were significantly higher in trained vs. untrained mice (p < 0.05). The data indicate significant dysfunction of lactate metabolism in ALS mice, associated with a reduction in muscle anaerobic metabolism and NADH transporting enzymes, as well as swim-induced compensation of energy demands in the ALS mice.