Background: Sexually transmitted infection (STI) screening in pregnancy provides an excellent opportunity for secondary prevention. Objective: To document the epidemiology of HIV, hepatitis B, and syphilis among pregnant women at a Guatemalan national hospital. 2008, 33 (0.36%) were to HIV-infected mothers. Conclusion: These 3 STIs were uncommon in our population and did not increase in incidence during the study period. HIV maternal-to-child transmission (MTCT) prevention programs were feasible in our setting.