Hydrophobic interaction chromatography (HIC) is an important technique for protein purification, which exploits the separation of proteins based on hydrophobic interactions between the stationary phase ligands and hydrophobic regions on the protein surface. One way of enhancing the purification efficiency by HIC is the addition of short sequences of peptide tags to the target protein by genetic engineering, which could reduce the need for extra and expensive chromatographic steps. In the present work, a methodology for predicting retention times of cutinases tagged with hydrophobic peptides in HIC is presented. Cutinase from Fusarium solani pisi fused to tryptophan-proline (WP) tags, namely (WP)2 and (WP)4, and produced in Saccharomyces cerevisiae strains, were used as model proteins. From the simulations, the methodology based on tagged hydrophobic definition proposed by Simeonidis et al. (Phitagged), associated to a quadratic model for predicting dimensionless retention times, showed small differences (RMSE<0.022) between observed and estimated retention times. The difference between observed and calculated retention times being lower than 2.0% (RMSE<0.022) for the two tagged cutinases at three different stationary phases, except for the case of cut_(wp)2 in octyl sepharose-2 M ammonium sulphate. Therefore, we consider that the proposed strategy, based on tagged surface hydrophobicity, allows prediction of acceptable retention times of cutinases tagged with hydrophobic peptides in HIC.
The growth of cities, specifically in Chile, has not necessarily brought with it an improvement in quality of life and in relation to the mental health of the inhabitants. We address the conjunction of public spaces, interpersonal relationships, and quality of life, as it has not been sufficiently considered. Quality of life is closely linked with social and interpersonal relationships and with social integration, and these have been affected
El artículo propone identificar los componentes adhocráticos de la participación política de jóvenes en un grupo de estudio universitario chileno. A partir de las denominadas estructuras organizativas adhocráticas, se exponen, en particular, los orígenes y confluencias entre los integrantes del grupo de estudio, estructuras organizativas horizontales con liderazgos situacionales y la interseccionalidad adhocrática como punto de convergencia entre intereses asociados a la militancia, la formación académica y la experiencia profesional. El estudio es de tipo cualitativo, mediante entrevistas semiestructuradas efectuadas a cinco miembros del grupo de estudio. Entre los principales resultados destacan nuevas formas de estructura organizativa en un grupo con objetivos políticos en donde son protagonistas los intereses personales, articulados con objetivos colectivos; las relaciones de tipo horizontal basadas en la colaboración, que permiten la autorrealización personal a partir de la satisfacción de intereses de participación de carácter egosintónico, o bien, que permiten responder a necesidades e inquietudes individuales. Los hallazgos permiten complejizar el concepto de adhocracia y ampliar su campo de aplicación para llevarlo desde el ámbito de la psicología social de las organizaciones al campo de la comprensón de las organizaciones políticas juveniles no partidistas y autogestionadas.
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