Allium cepa (onion; Liliaceae family), herbaceous annual plant and reported Sulphur based amino acids with many minerals including vitamins. Inspite of various medicinal uses of this plant as food and also showed various beneficial effects. The antimicrobial activity of aqueous extract from Allium cepa (onion bulb) was evaluated against bacterial strains using disc diffusion method. In contrast, inflammatory response or inhibition of T cell antigenic response was evaluated using typhoid vaccine and swine flu vaccine in Swiss mice. These studies were conducted according to ethical guidelines and determining T cell proliferation including estimation of cytokines from cell culture supernatant and also measuring its hemolytic activity. The results showed that aqueous extract show the highest inhibition rate against Pseudomonas aeruginosa and Salmonella enteritidis. In mice model studies, aqueous extract at higher doses showed inhibition in antigen specific T cell proliferation and also declining in the level of IFN-gamma production but enhancement in IL-4 production. In addition, aqueous extract at higher concentration does not showed any hemolytic activity. Overall, this study provides evidence for the presence of secondary metabolites in aqueous extract that probably interferes with bacterial growth (antimicrobial) and also declining in antigenic specific proliferation (i.e. anti-inflammatory) which would prevent and inhibit initiation and progression of many diseases.
India is considered to be one of the rich repositories of medicinal and aromatic plants, which are mainly used as starting raw materials for drug manufacturing and perfumery products. More than thirty percent of various medicinal plant species are routinely used for various medicinal purposes. In this study, our major objective is to evaluate its and antioxidant potential of protein extracted from leaves of these two medicinal plants, i.e. and Syzygium . In order to achieve this objective, studies were conducted and extracted protein from leaves (using Tris and acetone) and confirming its protein content through the Lowry test. In addition, (using the standard vaccine, i.e. Typhoid vaccine) and antioxidant (using DPPH [1, 1-diphenyl-2-picrylhydrazyl] assay) based studies were evaluated using a variable concentration of protein. The results indicate that proteins showed effect against typhoid vaccine and also showed antioxidant effect as compared to control. In short, proteins serve as a natural antioxidant and also showed , which may be useful in preventing free radical-induced diseases and also claimed its properties as well. Overall, these studies reveal that protein possesses potential benefits in terms of (against specific protein antigen) and antioxidant effect.
Aim and objective: Pollen is reported in flowers and leaves of various medicinal plants and showed its beneficial effect on human health. The objective of our study is to evaluate the antioxidant effect of flowers (Calendula officinalis and Dianthus chinensis) especially pollen protein and also determining its immunogenic effect against specific protein (typhoid vaccine) antigen in human whole blood samples. Methods: In this study, analytical studies were conducted for estimating the protein content using Lowry test and qualitatively determined through high performance thin layer chromatography (HPLC). In addition, immunological studies were also conducted using pollen protein for evaluating its antioxidant potential (using DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical assay) and determined its immunogenic effect using typhoid vaccine antigen and determining its hemolytic effect in human whole blood samples. Results: This work demonstrated that pollen protein content (determined through lowry test) at higher concentrations exhibited a significant declining in antigen specific T cell proliferation using optimized dose of typhoid vaccine antigen in human whole blood samples. In addition, pollen protein showed antioxidant property as well and does not show any hemolytic effect. Conclusion: The current study showed that pollen protein from Calendula officinalis and Dianthus chinensis possesses antioxidant property and less immunogenic in terms of declining in antigen specific T cell proliferation rate.
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