Abstract Background: Human sperm cell counting analysis is of significant interest to biologists studying sperm function and to medical practitioners evaluating male infertility. Currently the analysis of this assessment is done manually by looking at the sperm samples through a phase-contrast microscope using expert knowledge to do a subjective judgement of the quality. Aims: to eliminate the subjective and error prone of the manual semen analysis and to avoid inter and intra-laboratory inconsistencies in semen analysis test results Methods: In this paper we introduce a technique for human sperm concentration. Its principle is based on the execution of three steps: The first step in unavoidable. It concerns the pretreatment of the human sperm microscopic videos which consists of a conversion of the RGB color space into the YCbCr space, the “Gaussian filtering” and the “discrete wavelet filtering”. The second step is devoted to the segmentation of the image into two classes: spermatozoas and the background. To achieve this, we used an edge detection technique “Sobel Contour detector”. The third step is to separate true sperm from false ones. It uses a machine learning technique of type decision trees that consist on two classes classification based on invariant characteristics that are the dimensions of the bounding ellipse of the spermatozoid head as well as its surface. Results: To test the robustness of our system, we compared our results with those performed manually by andrologists. After results analysis, we can conclude that our system brings a real improvement of precision as well as treatment time which make it might be useful for groups who intend to design new CASA systems. Conclusion: In this study, we designed and implemented a system for automatic concentration assessment based on machine learning method and image processing techniques.
Computer-assisted semen analysis systems insist on evaluating sperm characteristics. These systems afford capacity to study and evaluate sperm statistical and morphological characteristics such as concentration, morphology, and motility, which have an important role in diagnosis and treatment of male infertility. In this paper, the proposed algorithm allows the assessment of concentration and motility rate of sperms in microscopic videos. First, enhancement process is required because of microscopic images limitations such as low contrast and noises. Then, for true sperm recognition among noise and debris, a hybrid approach is proposed using a combination between segmentation techniques. After, the use of geometric features of the bounding ellipse of the sperm head led to define sperm concentration. Finally, inter-frame difference is applied for motile sperm detection. The proposed method was tested on microscopic videos of human semen; the performance of this method is analyzed in terms of speed, accuracy, and complexity. Obtained results during the experiments are very promising compared with those obtained by the traditional assessment, which is the most widely used and approved in the laboratories.
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