The isolation of axisocyanate-3 as a minor component of Acanthella cavernosa has been confirmed by spectroscopic characterisation of the metabolite, and by conversion of a mixture of axisothiocyanate-3 and axisocyanate-3 to their diethyl(thio)urea derivatives. The role of isocyanates in the formation of urea metabolites in sponges has been investigated through a model study with a menthyl-derived isocyanate.
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